Protein Info for PP_3415 in Pseudomonas putida KT2440

Annotation: Transcriptional regulator, LacI family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 PF00532: Peripla_BP_1" amino acids 70 to 317 (248 residues), 78.7 bits, see alignment E=1.1e-25 PF13407: Peripla_BP_4" amino acids 72 to 320 (249 residues), 58.4 bits, see alignment E=1.7e-19 PF13377: Peripla_BP_3" amino acids 178 to 339 (162 residues), 98.4 bits, see alignment E=9.7e-32

Best Hits

KEGG orthology group: K06145, LacI family transcriptional regulator, gluconate utilization system Gnt-I transcriptional repressor (inferred from 99% identity to ppf:Pput_2347)

Predicted SEED Role

"Gluconate utilization system Gnt-I transcriptional repressor" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HE5 at UniProt or InterPro

Protein Sequence (339 amino acids)

>PP_3415 Transcriptional regulator, LacI family (Pseudomonas putida KT2440)
MSRTGSRTTGRPTLAEVARLSGVSPITASRALRGVSTVAPDLVEKVIAAAANLGYVANPA
ARALASARSQSVVVLIPSLSNQLFIDTLEAIHEVMRPRGLEVLIGNYHYDLAEEENLIRN
YLAYQPCGILLTGFERSDAARQMLVASGVPCVHMMELNGEPGALSVGFSQHQAGRAAARH
LIERGRKRLAFIAAQLDPRVMQRAEGFRQALAEAGLQAAELEVLAPEPSSIALGSVLFSQ
LMQQAPDVDGIFFCNDDLAQGAVLQALRQGVEVPRQVAMVGFNDLPASAHMVPRLTSIRT
PRAAVGRGAAQALLALLDGKRVTNAQQDLGFELMVREST