Protein Info for PP_3360 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 transmembrane" amino acids 17 to 42 (26 residues), see Phobius details amino acids 64 to 85 (22 residues), see Phobius details amino acids 97 to 116 (20 residues), see Phobius details amino acids 136 to 158 (23 residues), see Phobius details amino acids 165 to 186 (22 residues), see Phobius details PF21088: MS_channel_1st" amino acids 145 to 184 (40 residues), 38.6 bits, see alignment 1.3e-13 PF00924: MS_channel_2nd" amino acids 185 to 252 (68 residues), 69.9 bits, see alignment E=2.3e-23 PF21082: MS_channel_3rd" amino acids 258 to 341 (84 residues), 25.1 bits, see alignment E=2.9e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3360)

Predicted SEED Role

"Putative inner membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HJ6 at UniProt or InterPro

Protein Sequence (374 amino acids)

>PP_3360 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MNDRIIAFFTETTVFGISILNLLVALTVATLVFLVARAAISFLMRRVRRWSEHEGPLSQI
MAKVLSGTSNFLLLLASLLVGLSMLDLPERWLTRVSSLWFVVAALQIGLWANRAIGLGLS
RYFARHRTDGLNQGSALATLSAWGARVLLWSVVVLAMLSNLGVNITAFVASLGVGGIAVA
LAVQNILGDLFASLSIAVDKPFEIGDFIVIGPLAGTVEHVGLKTTRIRSLGGEQIVMANA
SMISSTIQNYKRLQERRIVFEFGLSYDTPTEAVKKAPAIVEDAIKAQEQARFDRAHLRGF
GKEALEFECVYIVQDPGYNLYMDIQQAINFRLLERFSQIGAKFAVPVRAIKVTALPEASG
LHGQAREGLSIRGA