Protein Info for PP_3346 in Pseudomonas putida KT2440

Annotation: Nickel transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 273 TIGR02769: nickel import ATP-binding protein NikE" amino acids 2 to 265 (264 residues), 436.6 bits, see alignment E=1.7e-135 PF00005: ABC_tran" amino acids 28 to 179 (152 residues), 109.2 bits, see alignment E=1.3e-35

Best Hits

Swiss-Prot: 100% identical to NIKE_PSEPK: Nickel import ATP-binding protein NikE (nikE) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02032, peptide/nickel transport system ATP-binding protein (inferred from 100% identity to ppu:PP_3346)

MetaCyc: 46% identical to nickel ABC transporter ATP binding subunit NikE (Escherichia coli K-12 substr. MG1655)
7.2.2.i [EC: 7.2.2.i]; 7.2.2.- [EC: 7.2.2.i]

Predicted SEED Role

"Nickel transport ATP-binding protein NikE (TC 3.A.1.5.3)" in subsystem Transport of Nickel and Cobalt (TC 3.A.1.5.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.i

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HL0 at UniProt or InterPro

Protein Sequence (273 amino acids)

>PP_3346 Nickel transporter ATP-binding protein (Pseudomonas putida KT2440)
MSLLHVHHVGHRYRTGGLLRKRGWLQVLDGIDLQLHAGESIGLLGSSGSGKSTLARLLLG
LEKPAQGQVSFAGQDVSQLKGEQARAFQRTVQLVFQDAPGAFNPQRSIGWSIAEPLRHLT
DMDEAARQARTLALLEEMGLRAEHAQRLPQQLSGGQLQRANIARALAASPQLVVLDEALS
NLDRLLQLQILQQLEALRQRSGTAFVLISHDLSLVQYFCQRVVLLHGGRIVEERPVTHDL
CFAHPVGRQLQAAVLPVRPQRRPSPQGLPTAAH