Protein Info for PP_3326 in Pseudomonas putida KT2440

Annotation: conserved exported protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 240 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF10670: DUF4198" amino acids 30 to 212 (183 residues), 66.6 bits, see alignment E=1.8e-22

Best Hits

KEGG orthology group: K10094, nickel transport system substrate-binding protein (inferred from 100% identity to ppu:PP_3326)

Predicted SEED Role

"Additional periplasmic component NikK of nickel ECF transporter" in subsystem Transport of Nickel and Cobalt

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HM7 at UniProt or InterPro

Protein Sequence (240 amino acids)

>PP_3326 conserved exported protein of unknown function (Pseudomonas putida KT2440)
MHPSLKVASLLALMVSVNAGAHGLWTEERRGNIEAVYGHGAEDNAYKAEKIKRAWAFDVT
GNMIPVTVERLADHAVLQPLTPPAALAVLLDNGPWSQRPDKQWVNQDRTHVKDAIASTHS
LKYSLAIYQSGARLPKLDMLRLAIVPLSDPLQVGPGKTLEVQVLLDGKPAADIPLQGDYR
GAPHQVSATTDKQGRARITVRNEGLNIIAAQATLPMADGSPVAQQSLFSSLSFLGKPHHE