Protein Info for PP_3319 in Pseudomonas putida KT2440

Annotation: Sensory box protein/GGDEF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 PF01590: GAF" amino acids 30 to 171 (142 residues), 45.6 bits, see alignment E=3.7e-15 PF13185: GAF_2" amino acids 32 to 171 (140 residues), 49.9 bits, see alignment E=1.4e-16 TIGR00229: PAS domain S-box protein" amino acids 181 to 305 (125 residues), 59.5 bits, see alignment E=3.7e-20 PF13188: PAS_8" amino acids 185 to 217 (33 residues), 17.2 bits, see alignment (E = 1.3e-06) PF00989: PAS" amino acids 186 to 295 (110 residues), 31.5 bits, see alignment E=5.4e-11 PF08448: PAS_4" amino acids 195 to 299 (105 residues), 34.4 bits, see alignment E=7.9e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 307 to 468 (162 residues), 143.9 bits, see alignment E=3.9e-46 PF00990: GGDEF" amino acids 309 to 466 (158 residues), 162 bits, see alignment E=3.6e-51 PF00563: EAL" amino acids 485 to 721 (237 residues), 259.3 bits, see alignment E=1.1e-80

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3319)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HN4 at UniProt or InterPro

Protein Sequence (733 amino acids)

>PP_3319 Sensory box protein/GGDEF domain protein (Pseudomonas putida KT2440)
MNTSCTSYHSMALSESQRDILGMIATNHQLHDILTAICLMIDAQDPGTLCSILLTDAQGK
HLLKGAAPGLPPAYSDAIHGLPIGPQEGTCGTAAFRRELVVTADIARDPSWERFRSLALG
YNLRSCWSLPLLSHEGSVLGTFALYQDQAIAPSEVQIHRLTCAAQLAAIAIRHERDGQRL
EESEQRFRSLFTYNPNPVFALDRAGNIQSVNPAGLKLKAGAATDLVGHHFSHLVFEEDLP
KVNQHFFAARAGEPQRFEARVRDESDDKQLIMDIANLPIMVNGEIVGVFGIARDISKQKH
YERQLSINASHDGLTGLLNRLSLEDRLVQACRSSRQRKVQLAVMYIDLDGFKSINDSIGH
YFGDQVLIEVAQRLTQQVRPADTVVRMGGDEFVVLLTDLLRDEDAVPVAERLLASIGKPY
CIQGIDLHVSTSIGITVSDGHIEQPMKLIQQADMAMYKAKQQGRNNFQWYTSDLNHRVCE
HRSLRNDLQKAIKTQSFSLHYQPQIEARTRRVVGIEALLRWEHPVRGFISPAVFIPVAED
SGQIIPLSLWVLDTACAQLRDLCDQGFNGISMAVNISPMHFQCGQFVECIQAVLSKHGLS
AGQLELEITESLLLHNVEQAIATLHRLKDLGVRIALDDFGTGFSSLSYLKCLPIDKIKID
RSFIQDISTNPHDAAITQGIISMAHHLSLSVVAEGVETAPQVNFLERGRCDILQGYYFAR
PMSHEALRTFLAD