Protein Info for PP_3311 in Pseudomonas putida KT2440

Annotation: glutathione-regulated potassium/H+ antiporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 581 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 34 to 53 (20 residues), see Phobius details amino acids 59 to 78 (20 residues), see Phobius details amino acids 90 to 109 (20 residues), see Phobius details amino acids 115 to 137 (23 residues), see Phobius details amino acids 155 to 175 (21 residues), see Phobius details amino acids 187 to 208 (22 residues), see Phobius details amino acids 227 to 255 (29 residues), see Phobius details amino acids 273 to 291 (19 residues), see Phobius details amino acids 298 to 320 (23 residues), see Phobius details amino acids 363 to 383 (21 residues), see Phobius details PF00999: Na_H_Exchanger" amino acids 17 to 375 (359 residues), 203.9 bits, see alignment E=5.7e-64 TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 18 to 290 (273 residues), 265.6 bits, see alignment E=2.8e-83 PF02254: TrkA_N" amino acids 407 to 521 (115 residues), 77 bits, see alignment E=2.2e-25

Best Hits

KEGG orthology group: K03455, monovalent cation:H+ antiporter-2, CPA2 family (inferred from 100% identity to ppu:PP_3311)

Predicted SEED Role

"putative Glutathione-regulated potassium-efflux system protein KefB" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HP2 at UniProt or InterPro

Protein Sequence (581 amino acids)

>PP_3311 glutathione-regulated potassium/H+ antiporter (Pseudomonas putida KT2440)
MAAEGNAGLLLGVITLLSAAVVAVPLLKRIGFGSVLGYLLAGMMIGPFGLQLVNDPHTII
DVGELGVVMFLFVIGLELNPSHLWALRAQIFGLGSLQVLICAVLLTVVGKAFGFPWQVSF
VCAAGFVLTSTAIVMQVLSERGDLTEPRGQRIVSILLFEDLLIVPLLAVVAFLAPTAAVP
EPTSPLWQRMAIAAVSLSALVIIGLWLLNPLFRVLAQARAREVMTAAALLVVLGAALLME
IGGLSMAMGAFVAGVLLSESTFRHQLEVDIEPFRGLLLGLFFLGVGMALDLQVVARNWLL
ISSGVLALMVVKALCIYGVARLAKSQHGDALDRAVLMAQGGEFAFVLFTEALKLKVVSAE
INANMTAIVVLSMALTPVNLLLLKRFARPRAVSMDGVEPAQNLQGHVLIIGFGRVGQIAC
QAPLAHGAKLSIIDMDPEVIRAVEPYGFKVYYGDGARHDLLHAAGARHARTIIVCVNDKK
AATRIVESTRQYCPQVKVLVRAFDREHALELVKHDADYIVRETFESSLLLGRQAVLTLGA
SSVEADAVTDEVRTRCRTFCPGNIRRSICRASVGSGKYRAH