Protein Info for PP_3270 in Pseudomonas putida KT2440
Annotation: oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to PAAZ_ECOLI: Bifunctional protein PaaZ (paaZ) from Escherichia coli (strain K12)
KEGG orthology group: K02618, phenylacetic acid degradation protein (inferred from 100% identity to ppu:PP_3270)MetaCyc: 59% identical to fused 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase and oxepin-CoA hydrolase (Escherichia coli K-12 substr. MG1655)
3-hydroxybutyryl-CoA dehydratase. [EC: 4.2.1.55]; RXNMETA-12671 [EC: 4.2.1.55, 3.3.2.12]; RXNMETA-12672 [EC: 4.2.1.55, 3.3.2.12, 1.2.1.91]
Predicted SEED Role
"Phenylacetic acid degradation protein PaaN, ring-opening aldehyde dehydrogenase (EC 1.2.1.3)" (EC 1.2.1.3)
MetaCyc Pathways
- superpathway of phenylethylamine degradation (11/11 steps found)
- phenylacetate degradation I (aerobic) (9/9 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- octane oxidation (4/5 steps found)
- L-tryptophan degradation X (mammalian, via tryptamine) (3/4 steps found)
- phytol degradation (3/4 steps found)
- putrescine degradation III (3/4 steps found)
- ethanol degradation III (2/3 steps found)
- hypotaurine degradation (2/3 steps found)
- (2S)-ethylmalonyl-CoA biosynthesis (2/4 steps found)
- fatty acid α-oxidation I (plants) (2/4 steps found)
- acetyl-CoA fermentation to butanoate (4/7 steps found)
- serotonin degradation (4/7 steps found)
- histamine degradation (1/3 steps found)
- 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
- noradrenaline and adrenaline degradation (8/13 steps found)
- dopamine degradation (2/5 steps found)
- aromatic biogenic amine degradation (bacteria) (3/8 steps found)
- ceramide degradation by α-oxidation (2/7 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- alkane oxidation (1/6 steps found)
- limonene degradation IV (anaerobic) (1/7 steps found)
- ethylmalonyl-CoA pathway (2/11 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
KEGG Metabolic Maps
- 1,2-Dichloroethane degradation
- 3-Chloroacrylic acid degradation
- Arginine and proline metabolism
- Ascorbate and aldarate metabolism
- Butanoate metabolism
- Fatty acid metabolism
- Glycerolipid metabolism
- Glycolysis / Gluconeogenesis
- Histidine metabolism
- Limonene and pinene degradation
- Lysine degradation
- Propanoate metabolism
- Pyruvate metabolism
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.3
Use Curated BLAST to search for 1.2.1.3 or 1.2.1.91 or 3.3.2.12 or 4.2.1.55
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88HT3 at UniProt or InterPro
Protein Sequence (684 amino acids)
>PP_3270 oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (Pseudomonas putida KT2440) MSAAPTLQSFIAGRWLGQHGAQALRSALDGHVLAYSHEERPDFAEAVDYARARGLASLMG MDFQQRAQRLKALALYLAECKEQLYALSHHSGATRADSWIDIEGGNATLFSYAGIGSREL PSGNLVHEGPAIPLGKQGHFAGSHILVPRAGVAVHINAFNFPIWGMLEKFAPTFLAGMPC IVKPATSTSYLTEAVVRLMNASGLLPEGSLQLVIGSTGDLLDRLQGQDVVTFTGSADTAA KLRVTPNLIRNSVPFTAEADSLNCAILGPDVSPDSEEFDLYIKEVVREMTTKAGQKCTAI RRAIVPARHLDAVATRLRERLSKVVVGDPSLEGVRMGALASHDQQRDVGERVRSLLQSCD QLFGASDGFAPRGEGVAEGAFFAPTLLQARDPHAEGGAHDIEAFGPVSTLMAYDDLDEAL ALAARGKGSLVATLVTADRSVAAKAIPVAAAWHGRLLVLDSQAAKESTGHGSPLPQLKHG GPGRAGGGEELGGLRAVKHYLQRAAVQGSPSMLTAVTGEYVRGGEVIETEVHPFRRYFEQ LRVGESLLTHRRTVTEADLVNFGCLSGDHFYMHFDEIAAKASQFGKRIAHGYFVLSAAAG LFVSPGAGPVLANYGLDTLRFINPVGIGDTIQARLTCKRKIDQGKTSPLGQPQGVVAWDV EVTNQLGELVASYDILTLVLKKPA