Protein Info for PP_3212 in Pseudomonas putida KT2440
Annotation: Rieske 2Fe-2S family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 100% identity to ppu:PP_3212)Predicted SEED Role
"Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82)" in subsystem Phenylpropanoid compound degradation (EC 1.14.13.82)
MetaCyc Pathways
- vanillin and vanillate degradation II (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.14.13.82
Use Curated BLAST to search for 1.14.13.82
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88HZ0 at UniProt or InterPro
Protein Sequence (341 amino acids)
>PP_3212 Rieske 2Fe-2S family protein (Pseudomonas putida KT2440) MLVTKQPVLRRFWYALLPMTALEDGPKPFTLLGEALVLWKRPDGTPVAMRDRCCHRTAKL SKGFVSAEGNIACGYHGWEYDCTGTCVKIPQSPTGAIPPGAAVKAYHCQEKYGYAWVSLD DPLQPIPEFPEDGAPGYRRIFQFYEEWKTSPLRVMENSFDNSHFSFVHKANFGLFDQPQP ASYEFRETDYGFEAETRVPIRNPEASFRITGTTEPITQRHLLNRYYLPFSRRFGCMYPDS GIHHIIYNCATPIDDNRLMLVQWLYRNDSEEQCSTQELIDWDAAITAEDRDILEATDPDA CVDTRRRVELHMPSDKPGLVIRRQLLGLLEAHGESEVFLSH