Protein Info for PP_3189 in Pseudomonas putida KT2440

Annotation: cytosine deaminase / isoguanine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 PF07969: Amidohydro_3" amino acids 43 to 382 (340 residues), 163 bits, see alignment E=1.8e-51 PF01979: Amidohydro_1" amino acids 49 to 378 (330 residues), 45.9 bits, see alignment E=4.8e-16

Best Hits

Swiss-Prot: 59% identical to CODA_ECOLI: Cytosine deaminase (codA) from Escherichia coli (strain K12)

KEGG orthology group: K01485, cytosine deaminase [EC: 3.5.4.1] (inferred from 100% identity to ppu:PP_3189)

MetaCyc: 75% identical to cytosine deaminase monomer (Klebsiella pneumoniae)
Cytosine deaminase. [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]

Predicted SEED Role

"Cytosine deaminase (EC 3.5.4.1)" (EC 3.5.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88I13 at UniProt or InterPro

Protein Sequence (413 amino acids)

>PP_3189 cytosine deaminase / isoguanine deaminase (Pseudomonas putida KT2440)
MNIINARLRGKPGLFRVELNGERIAAIVPQTDVVASATANDLDAGQNLVVAPFVEPHIHL
DATLTAGEPKWNMSGTLFEGIERWSERKEITTQDDIKTRAKKTIGMLVDHGIQHVRTHVD
VTDPKLTALKVMVEVRDEVRELIDLQIVAFPQEGIESYANGRALMTEAVAIGADVVGGIP
HFENTRDQGVSSVKFLMDLAERTGCLVDVHCDETDDPQSRFLEVLAEEARVRGMGARVTA
SHTVAMGSYDNAYCYKLFRLLKLSGINFVSCPTESIHLQGRFDNYPKRRGVTRVAEIDRA
GMNVCFGQDSIVDPWYPLGNGNILRILEAGLHICHMLGYEDLQRCLDLITDNSARTLNLG
ERYGIEVGRPANLLLLSAPDDYEMVRSQGHALVSVRNGKVLMQRTPAQVQRLR