Protein Info for PP_3173 in Pseudomonas putida KT2440

Annotation: N-ethylmaleimide reductase, FMN-linked

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00724: Oxidored_FMN" amino acids 2 to 340 (339 residues), 272.2 bits, see alignment E=3.6e-85

Best Hits

Swiss-Prot: 70% identical to NEMA_ECOLI: N-ethylmaleimide reductase (nemA) from Escherichia coli (strain K12)

KEGG orthology group: K10680, N-ethylmaleimide reductase [EC: 1.-.-.-] (inferred from 100% identity to ppu:PP_3173)

MetaCyc: 70% identical to N-ethylmaleimide reductase (Escherichia coli K-12 substr. MG1655)
1.3.1.-

Predicted SEED Role

"N-ethylmaleimide reductase (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88I29 at UniProt or InterPro

Protein Sequence (365 amino acids)

>PP_3173 N-ethylmaleimide reductase, FMN-linked (Pseudomonas putida KT2440)
MKLLQPLQIGPLTLPNRVFMAPLTRLRSLEPGDVPTALMAEYYRQRASAGLIITEATQIS
FQAKGYSGSPGIHSAEQIAAWKHINEGIHAEGGHSAVQVWHTGRVSHTSLQPGGKAPVAP
SALPADARTTLRDAQGNLTRVETSAPRALSEAEIAGIVADFGQAAINACEAGFDFIELHA
AHGYLLHQFLTPSANQREDRYGGSVENRARIVLEAVDAAIANWSADRVGIRVFPLGGFNG
VDNGEDQEAAGLYLIRELAKRNLAYLHLSEPDWAGGKPLRDEFRQAIRAAYPGVIIAAGA
YTAEKGEDLIGRGLIDAVAFGRAYIANPDLVERLRLQAPLNEHRAKFDYANGPEGYTDYP
FLKQA