Protein Info for PP_3126 in Pseudomonas putida KT2440

Annotation: putative polysaccharide exported protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF02563: Poly_export" amino acids 23 to 97 (75 residues), 82.1 bits, see alignment E=3.8e-27 PF10531: SLBB" amino acids 104 to 154 (51 residues), 32 bits, see alignment E=1.3e-11 PF22461: SLBB_2" amino acids 105 to 184 (80 residues), 41.2 bits, see alignment E=2.2e-14

Best Hits

KEGG orthology group: K01991, polysaccharide export outer membrane protein (inferred from 100% identity to ppu:PP_3126)

Predicted SEED Role

"Capsular polysaccharide biosynthesis/export periplasmic protein WcbA; Capsular polysaccharide export system protein KpsC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88I75 at UniProt or InterPro

Protein Sequence (185 amino acids)

>PP_3126 putative polysaccharide exported protein (Pseudomonas putida KT2440)
MVRSMFAAFCMLTVWSGAGNADQSSAAYLLSPGDVVMVSVWQEESLRQEATVLPDGSITF
PLAGRIDVAGLDVTAVEKQVAAKLEKYLPDPNVSVVVKSIAGNMVYVQGKVIKPGPVQMA
GPTAVLQALSMSGGLDKFADESEIKVVRGTGASQKILPVRYKDLVSGRDMSTNIQLQAGD
TLVVP