Protein Info for PP_3116 in Pseudomonas putida KT2440

Annotation: transcriptional repressor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 PF01726: LexA_DNA_bind" amino acids 1 to 65 (65 residues), 62.7 bits, see alignment E=2.2e-21 TIGR00498: repressor LexA" amino acids 1 to 201 (201 residues), 179.8 bits, see alignment E=2.6e-57 PF00717: Peptidase_S24" amino acids 81 to 195 (115 residues), 116.2 bits, see alignment E=6.7e-38

Best Hits

Swiss-Prot: 100% identical to LEXA2_PSEPK: LexA repressor 2 (lexA2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01356, repressor LexA [EC: 3.4.21.88] (inferred from 100% identity to ppf:Pput_2601)

Predicted SEED Role

"SOS-response repressor and protease LexA (EC 3.4.21.88)" in subsystem DNA repair, bacterial (EC 3.4.21.88)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.88

Use Curated BLAST to search for 3.4.21.88

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P59479 at UniProt or InterPro

Protein Sequence (202 amino acids)

>PP_3116 transcriptional repressor (Pseudomonas putida KT2440)
MDTLTPKRRAIFEFIRERIAEHGQPPSLADIATRFGFASRSVARKHITALCQAGYIDVTP
NQARGIRLAEPLRRPEILEIPVLGQVAAGAPIGPDLGIHEQLLLDPSLFRRTPDYLLKVR
GDSMIDDGIFDGDLVGILQQADARDGQIVVARLDGEVTIKRLQRQGGTYRLLPRNPAYAP
IDVQPEQDFFIEGVFCGLLRRD