Protein Info for PP_3076 in Pseudomonas putida KT2440

Annotation: putative ABC transporter permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details transmembrane" amino acids 143 to 166 (24 residues), see Phobius details amino acids 178 to 197 (20 residues), see Phobius details amino acids 203 to 221 (19 residues), see Phobius details amino acids 260 to 285 (26 residues), see Phobius details amino acids 307 to 330 (24 residues), see Phobius details PF12911: OppC_N" amino acids 7 to 42 (36 residues), 26.6 bits, see alignment 4.4e-10 PF00528: BPD_transp_1" amino acids 157 to 337 (181 residues), 106.4 bits, see alignment E=1.6e-34

Best Hits

Swiss-Prot: 60% identical to YEJE_ECOLI: Inner membrane ABC transporter permease protein YejE (yejE) from Escherichia coli (strain K12)

KEGG orthology group: K13895, microcin C transport system permease protein (inferred from 100% identity to ppu:PP_3076)

MetaCyc: 60% identical to putative oligopeptide ABC transporter membrane subunit YejE (Escherichia coli K-12 substr. MG1655)
3.6.3.23-RXN [EC: 7.4.2.6]

Predicted SEED Role

"Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) (TC 3.A.1.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IC3 at UniProt or InterPro

Protein Sequence (338 amino acids)

>PP_3076 putative ABC transporter permease protein (Pseudomonas putida KT2440)
MLSPLSRRRLQRFRRHRLGWVSLWLFAGLLLLSLCAELVANDKPLLLGYKGNVYVPALKR
YTEQQFGGQLPFQPDYRSAYVRQLIAEQGGWMLFAPIPFSADTPNYDLQVPTPSPPSASN
WLGTDDQGRDVLARVLYGTRVSLLFAFALTVVSVLIGVAAGALQGYHGGWVDLFGQRLLE
VWSGLPVLYLLIILSGFVEPDFWWLLGIMALFSWLTLVDVVRAEFLRGRNLEYVKAARAL
GLPDSQVMLRHILPNAMNATLTYVPFMLTGAITTLTALDFLGFGMPAGSASLGELVTQGK
QHLEAPWLGFTAFFALAVILSLLVFIGDALREAFDPRR