Protein Info for PP_3072 in Pseudomonas putida KT2440

Annotation: Ecotin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 159 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF03974: Ecotin" amino acids 28 to 149 (122 residues), 176.2 bits, see alignment E=1.2e-56

Best Hits

Swiss-Prot: 100% identical to ECOT_PSEPK: Ecotin (eco) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K08276, ecotin (inferred from 99% identity to ppf:Pput_2652)

Predicted SEED Role

"Proteinase inhibitor I11, ecotin precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IC7 at UniProt or InterPro

Protein Sequence (159 amino acids)

>PP_3072 Ecotin (Pseudomonas putida KT2440)
MRPTPMTAILALSLAAAAPAMAASLKDIAPYPEAEKGFTRQVIHLPAQADESAYKLEILA
GKTLQVDCNRQRLGGNLEAHTLEGWGYNYYRLDNVSGPASTLMACPDGKKTEAFVPVVGD
GFLLRYNSKLPVVVYVPKDVEVRYRVWSASQDVQKAKVE