Protein Info for PP_3071 in Pseudomonas putida KT2440

Annotation: acetoacetyl-coenzyme A synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 transmembrane" amino acids 316 to 335 (20 residues), see Phobius details TIGR01217: acetoacetate-CoA ligase" amino acids 3 to 650 (648 residues), 886.8 bits, see alignment E=3.8e-271 PF16177: ACAS_N" amino acids 36 to 91 (56 residues), 53.2 bits, see alignment 3.3e-18 PF00501: AMP-binding" amino acids 95 to 469 (375 residues), 159.9 bits, see alignment E=1.3e-50 PF13193: AMP-binding_C" amino acids 542 to 612 (71 residues), 24.6 bits, see alignment E=5.8e-09

Best Hits

Swiss-Prot: 47% identical to AACS_XENTR: Acetoacetyl-CoA synthetase (aacs) from Xenopus tropicalis

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 100% identity to ppu:PP_3071)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IC8 at UniProt or InterPro

Protein Sequence (650 amino acids)

>PP_3071 acetoacetyl-coenzyme A synthetase (Pseudomonas putida KT2440)
MNDVLWRPSTTQIEASRMDAFRRRVNLRYNLQLNDYQALHGWSIEQRPAFWQTLAEYFQV
HWHTPPSQVLSEGPQMPDAQWFANATLNFAEHLLRRRDDRPAVVAVREDGQREVFTHAEL
AAQVAGLQTAFKAVGIVPGDRVAAIMPNTWQTLVAMLACTSLGAVWSTSSPEFGVHGIID
RFGQIEPKLLIVCAGYQYAGKAIDQVTKVNQVCAQLPGLEHLIVVPHTRKGTRADEFQAA
NVSLWNDFFQPGGEPRFTPLPFDHPLYILYSSGTTGVPKCIVHRAGGVLLQHLKEHGLHN
DLKADDVLFYYTTCGWMMWNWLASGLAVGATLVLYDGSPFHPGPERLLDLIEAEGIHAFG
TSAKYLAALEQAGLEPANSHCLTSLRLLLSTGSPLSPHSYDYVYDKIKADLWLVSMSGGT
DIVSCFVLGNPTLPVRRGEIQCKGLGMAVEVWNEQGQPVVDEKGELVCTRNFPSMPLGFW
NDPDGRRYHDAYFSQFPGVWAQGDYAEQRASGGMVIHGRSDAVLNPGGVRIGTAEIYRQV
EKVEQVVESVAIGQDWNGDVRVVLFVRLQDGLQLDEALRQHIRQVIRQYTTPRHVPAVIA
QVSDIPRTISGKLVELAIRDVVHGRPVKNTDALANPEALEQFRDRAELKS