Protein Info for PP_3029 in Pseudomonas putida KT2440

Annotation: DNA cytosine methyltransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 609 PF00145: DNA_methylase" amino acids 25 to 139 (115 residues), 38.5 bits, see alignment E=4.8e-14

Best Hits

KEGG orthology group: K00558, DNA (cytosine-5-)-methyltransferase [EC: 2.1.1.37] (inferred from 100% identity to ppu:PP_3029)

Predicted SEED Role

"DNA cytosine methyltransferase family protein"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.37

Use Curated BLAST to search for 2.1.1.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IH0 at UniProt or InterPro

Protein Sequence (609 amino acids)

>PP_3029 DNA cytosine methyltransferase family protein (Pseudomonas putida KT2440)
MTVAQQISLEGAQQHSLPFSRELYVDLFAGAGGASSGGARAYRDPDIAINHNPIAIAVHR
ANHRKTRHYICDIYEVDPLEATGGQPVGILWASPDCRHFSKAKGGAPRSKAVRSLPWVVV
HWVFATRPRLLLMENVEEFQAWGPLNDEGKPIKSEMGRTFKAFVACLTTGLPADHPDMSE
VMDCIGQWVPMDALVRGLGCNVEWRERRASNAGSPTIRKRLFLIGRTDGRPIVWTKPKRH
ENPKPGQLPWRTAAECIDFSDLGKSLFARKRPLVDNTCRRVAKGFWRHTVMAEQPFVLQL
DEQKLAAASLTEFANASTQRTFSAGEPLRTQVAQIKGGHFALAAAHLTHLTHHGDRSGYP
VSEPTRTITGANRGEQALITASMITLRKGSTSSGMDHPVNALTTGSGHHAVAACHFEQAN
GGFYKGDGRAAKVPLSTILGRGTNQRLASAYLVKYYGTGGQWQDMGEPMHTLPTKERMAL
VTVVQVPAAILPPELLVKARKCAHFLHKYLPEHFPDLVDLVLLGEHALVDFTLRMLKAPE
LKLAQGFSPDYILDRGLFENQETGHLEWRPINKTDQIRLIGNSVCPDEAEDLIAANAKDL
IDLYQQEAA