Protein Info for PP_2977 in Pseudomonas putida KT2440

Annotation: Tn4652, transposase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 transmembrane" amino acids 145 to 163 (19 residues), see Phobius details PF13276: HTH_21" amino acids 49 to 104 (56 residues), 70.9 bits, see alignment E=1.7e-23 PF00665: rve" amino acids 129 to 227 (99 residues), 97.3 bits, see alignment E=1.1e-31 PF13683: rve_3" amino acids 217 to 283 (67 residues), 40 bits, see alignment E=5.4e-14 PF13333: rve_2" amino acids 236 to 287 (52 residues), 27.2 bits, see alignment 7.1e-10

Best Hits

Swiss-Prot: 60% identical to TRA9_MYCTO: Putative transposase for insertion sequence element IS986/IS6110 (MT1803) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2977)

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IM2 at UniProt or InterPro

Protein Sequence (301 amino acids)

>PP_2977 Tn4652, transposase subunit B (Pseudomonas putida KT2440)
MIWQLIDKHRQSFGVEPLCRVLQVSPSAYRRHAARLRDRSRRSARAIRDEELAVQIHRVW
QENYEVYGARKVWRQMHREHQVVARCTVERLMREMGLCGVTRGKTVKTTQPDPDNANPRD
LVKRQFTAERPNQLWVADFTFVSTWQGFAYVAFIVDVYSRFIVGWRVSRHMRTEFVLDAL
EQALHTRRPEPHRLVHHSDRGSQYLSIRYSERLGEAGIEPSVGNTGDSYDNALAETINGL
YKTELIHKRGPWKSVDSLEWETLKWVTWFNHQRLLEPIGNRPPAEFEALYEQSQATISVA
A