Protein Info for PP_2935 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 transmembrane" amino acids 19 to 42 (24 residues), see Phobius details amino acids 49 to 68 (20 residues), see Phobius details amino acids 88 to 108 (21 residues), see Phobius details amino acids 114 to 140 (27 residues), see Phobius details amino acids 152 to 173 (22 residues), see Phobius details amino acids 179 to 197 (19 residues), see Phobius details amino acids 218 to 245 (28 residues), see Phobius details amino acids 252 to 271 (20 residues), see Phobius details amino acids 280 to 300 (21 residues), see Phobius details amino acids 306 to 328 (23 residues), see Phobius details amino acids 342 to 363 (22 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details PF07690: MFS_1" amino acids 23 to 251 (229 residues), 51.6 bits, see alignment E=3.6e-18 amino acids 225 to 393 (169 residues), 45.3 bits, see alignment E=3e-16

Best Hits

Swiss-Prot: 55% identical to Y3873_PSEAB: Uncharacterized MFS-type transporter PA14_38730 (PA14_38730) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2935)

Predicted SEED Role

"transporter, putative"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IR4 at UniProt or InterPro

Protein Sequence (397 amino acids)

>PP_2935 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MTAQQPAPASHTLHITLQILSIVFYTFIAFLCIGLPIAVLPSYVHDQLGFGAVIAGVTIG
LQYLATLLSRPFAGRVADTLGGKQAIRFGLLGIAGCGVLTLLSAWTLTLPLLSLALLLGG
RLLLGIAQGLIGVATLSWGISQVGPVHTARVISWNGIASYGAIAIGAPVGVLAVDGLDFS
VLGPALLVLATLALLVLRKRPDVVVVRGERLPFWSAFGRVAPCGLGLTLASIGYGTLTTF
VTLYYLERGWAGAAWCLSAFGVCFIISRLLFVNAVNRFGGYNVAVACMATEVLGLSLLWL
APSPPWALVGAGLTGFGLSLVYPALGVEAIKQVPSSSRGAGLGAYAVFFDMALAIAGPVM
GAVAVHLGYASIFCVAALLALAGVGLTLWLARRARRS