Protein Info for PP_2925 in Pseudomonas putida KT2440

Annotation: malate:quinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 30 to 516 (487 residues), 773.7 bits, E=6.4e-237
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 31 to 514 (484 residues), 687.6 bits, E=4.7e-211
• PF01266: DAO
  • amino acids 32 to 302 (271 residues), 31.5 bits, E=1.4e-11

Best Hits

Swiss-Prot: 100% identical to MQO3_PSEPK: Probable malate:quinone oxidoreductase 3 (mqo3) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 100% identity to ppu:PP_2925)

MetaCyc: 66% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Sequence Analysis Tools

See Q88IS4 at UniProt or InterPro

Protein Sequence (539 amino acids)

>PP_2925 malate:quinone oxidoreductase (Pseudomonas putida KT2440)
MKITIKPKLAMAGLALAIGMLNAQAAETRKVDVLLVGGGIMSSTLAVWLNELEPGWSMEM
VERLDKVAEESSNGWNNAGTGHSALAELNYTPEKDGKIDISKAVEINESFQVTRQFLAWQ
VKQGVLKNPHSFINTTPHMSFVWGDDNIRFLKKRYEALQASPLFKPMQYSEDHEQIRKWV
PLMMEGRDPNQKLAVTWTPIGTDVNFGEITRQYVGYLQTRPNFDLKLSSEVQDITRNDDG
SWHVEYKNLKNGSKAATDARFLFIGAGGAALPLLQKSGIDEAKNYGGFPVGGSFLVTDNP
AIAQRHMAKAYGIAATGAPPMSVPHLDTRVLDGKRLILFGPFATFSTKFLKQGSLLDLLA
STSVHNAWPMVRVGVREFDLVQYLIGQVLQSDDDRFEALRTYFPEAKKEDWRLWQAGQRV
QIIKKDEALGGVLKLGTEVVTSRDGSIAGLLGASPGASTAAPIMLDVLNKVFKNKVASPE
WQAKIKQIIPSYGIRLNDHPDQLEKEWAYTNEVLQLEPPVSPQRPESIRPADSQGVASR