Protein Info for PP_2914 in Pseudomonas putida KT2440

Annotation: osmosensory proline/betaine/H+ permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 transmembrane" amino acids 32 to 52 (21 residues), see Phobius details amino acids 64 to 87 (24 residues), see Phobius details amino acids 99 to 118 (20 residues), see Phobius details amino acids 123 to 147 (25 residues), see Phobius details amino acids 167 to 187 (21 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 261 to 279 (19 residues), see Phobius details amino acids 299 to 319 (21 residues), see Phobius details amino acids 328 to 347 (20 residues), see Phobius details amino acids 353 to 377 (25 residues), see Phobius details amino acids 389 to 412 (24 residues), see Phobius details amino acids 418 to 439 (22 residues), see Phobius details PF00083: Sugar_tr" amino acids 29 to 449 (421 residues), 245.2 bits, see alignment E=2.1e-76 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 34 to 433 (400 residues), 475.8 bits, see alignment E=6.1e-147 PF07690: MFS_1" amino acids 62 to 311 (250 residues), 83.3 bits, see alignment E=2.4e-27 amino acids 276 to 443 (168 residues), 50.8 bits, see alignment E=1.8e-17 PF08946: Osmo_CC" amino acids 455 to 500 (46 residues), 71.1 bits, see alignment 1.1e-23

Best Hits

Swiss-Prot: 84% identical to PROP_SALTY: Proline/betaine transporter (proP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 100% identity to ppf:Pput_2777)

MetaCyc: 83% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88IT5 at UniProt or InterPro

Protein Sequence (500 amino acids)

>PP_2914 osmosensory proline/betaine/H+ permease (Pseudomonas putida KT2440)
MKPRKKSVQPIGLKDITIVDDAKMKKAITAAALGNAMEWFDFGVYGFVAYALGKVFFPNA
DPSVQMIAALATFSVPFLIRPLGGLFFGALGDRFGRQKILAATIVIMSLSTFAIGLIPSY
ASIGIWAPILLLLAKMAQGFSVGGEYTGASIFVAEYAPDRKRGFLGSWLDFGSIAGFVLG
AGVVVLISTVLGEEKFLDWGWRLPFFLALPLGIIGLYLRHALEETPAFQQHVDKLEQGDR
EGLASGPKVSFKEVATQHWRSLVTCIGVVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVL
IIIAIMVGMLFVQPIIGLLSDKFGRRPFIIVGSVGLLALAIPAFMLINSGVLGLIFAGLL
IIAVLLNFFIGVMASTLPAMFPTHIRYSALASAFNISVLIAGLTPTLAAWLVETTNDLYM
PAYYLMVIAAVGLVTGLTMKETANKPLRGAAPAASDIEEARELLQEHHDNIEQKIEDIDA
QIAELEAKREKLALQHPRIE