Protein Info for PP_2799 in Pseudomonas putida KT2440
Annotation: Aminotransferase, class III
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to GATP1_ORYSJ: Gamma-aminobutyrate transaminase 1, mitochondrial (OSL2) from Oryza sativa subsp. japonica
KEGG orthology group: None (inferred from 100% identity to ppu:PP_2799)MetaCyc: 50% identical to gamma aminobutyrate transaminase (Solanum lycopersicum)
RXN-6902 [EC: 2.6.1.96]
Predicted SEED Role
"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)
MetaCyc Pathways
- biotin biosynthesis I (13/15 steps found)
- biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
- 4-aminobutanoate degradation IV (2/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.62
Use Curated BLAST to search for 2.6.1.62 or 2.6.1.96
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88J50 at UniProt or InterPro
Protein Sequence (459 amino acids)
>PP_2799 Aminotransferase, class III (Pseudomonas putida KT2440) MSTHSSTVQNDLAALIHPNTNLAQHREVGPLVIARGDGVRVFDEQGNAYIEAMSGLWSAA LGFSEQRLVDAAVEQFKQLPYYHSFSHKTNAPAAALAAKLAALAPGDLNHVFFTNSGSEA NDSVVKMVWYVNNALGRPAKKKFISRQQAYHGATVAAASLTGIPSMHRDFDLPAIPVHHL TCPNFYRFARPGESQEAFTVRLANELERYILAEGPETIAAFIGEPVIAAGGVIPPPTGYW AAIQAVCKRYDILVVIDEIITGFGRLGTMFGSQLYGIQPDIMVLSKQLTSSYQPLAAVVV SDAMNDVLVSQSQRLGAFAHGLTCTGHPVATAVALENIRIIEERDLVGHVQHLAPVFQRH LRAFEDHPLVGNVRGVGLMGGIELVADKATRQPFAQPGTLGGYVFKQAHKHGLIIRAIYD TIAFCPPLITTQDDIEAIFSAFERTLADATDWARSQHLL