Protein Info for PP_2795 in Pseudomonas putida KT2440
Annotation: AMP-binding domain protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 100% identity to ppu:PP_2795)MetaCyc: 100% identical to short chain acyl-CoA synthetase (Pseudomonas putida KT2440)
Butyrate--CoA ligase. [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- fatty acid salvage (6/6 steps found)
- valproate β-oxidation (7/9 steps found)
- octane oxidation (4/5 steps found)
- 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
- 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
- 4-oxopentanoate degradation (5/9 steps found)
- cannabinoid biosynthesis (6/13 steps found)
- sporopollenin precursors biosynthesis (4/18 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis II
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Butanoate metabolism
- Caprolactam degradation
- Ethylbenzene degradation
- Geraniol degradation
- Limonene and pinene degradation
- Propanoate metabolism
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.-
Use Curated BLAST to search for 6.2.1.- or 6.2.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88J54 at UniProt or InterPro
Protein Sequence (548 amino acids)
>PP_2795 AMP-binding domain protein (Pseudomonas putida KT2440) MMVPTLEHELAPNEANHVPLSPLSFLKRAAQVYPQRDAVIYGARRYSYRQLHERSRALAS ALERVGVQPGERVAILAPNIPEMLEAHYGVPGAGAVLVCINIRLEGRSIAFILRHCAAKV LICDREFGAVANQALAMLDAPPLLVGIDDDQAERADLAHDLDYEAFLAQGDPARPLSAPQ NEWQSIAINYTSGTTGDPKGVVLHHRGAYLNACAGALIFQLGPRSVYLWTLPMFHCNGWS HTWAVTLSGGTHVCLRKVQPDAINAAIAEHAVTHLSAAPVVMSMLIHAEHASAPPVPVSV ITGGAAPPSAVIAAMEARGFNITHAYGMTESYGPSTLCLWQPGVDELPLEARAQFMSRQG VAHPLLEEATVLDTDTGRPVPADGLTLGELVVRGNTVMKGYLHNPEATRAALANGWLHTG DLAVLHLDGYVEIKDRAKDIIISGGENISSLEIEEVLYQHPEVVEAAVVARPDSRWGETP HAFVTLRADALASGDDLVRWCRERLAHFKAPRHVSLVDLPKTATGKIQKFVLREWARQQE AQIADAEH