Protein Info for PP_2774 in Pseudomonas putida KT2440

Annotation: glycine betaine ABC transporter, ATPase/permease fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 transmembrane" amino acids 328 to 348 (21 residues), see Phobius details amino acids 354 to 375 (22 residues), see Phobius details amino acids 385 to 404 (20 residues), see Phobius details amino acids 424 to 454 (31 residues), see Phobius details amino acids 461 to 479 (19 residues), see Phobius details amino acids 484 to 503 (20 residues), see Phobius details amino acids 509 to 532 (24 residues), see Phobius details amino acids 544 to 566 (23 residues), see Phobius details amino acids 570 to 588 (19 residues), see Phobius details amino acids 626 to 652 (27 residues), see Phobius details amino acids 667 to 685 (19 residues), see Phobius details PF00005: ABC_tran" amino acids 56 to 203 (148 residues), 118.5 bits, see alignment E=5.1e-38 PF13304: AAA_21" amino acids 173 to 237 (65 residues), 27.7 bits, see alignment E=4.4e-10 PF00528: BPD_transp_1" amino acids 524 to 680 (157 residues), 82.8 bits, see alignment E=4e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2774)

Predicted SEED Role

"L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88J75 at UniProt or InterPro

Protein Sequence (698 amino acids)

>PP_2774 glycine betaine ABC transporter, ATPase/permease fusion protein (Pseudomonas putida KT2440)
MIADKYPQIANDPLETTARISADALGSVSIHDIALEFRAVTKRYEGSRSDSTNAVEQVNL
AVRRGEVLCLMGTSGSGKSTLLRHVNRLIEPSSGEVLIDGEVLSSLTQPTLRQLRSQRIG
MVFQHFGLLPHRSVLDNVALPLELRGEPESTRRTAALRQLQAVGLKAWSERFPHELSGGM
QQRVGLARALVTNPDILLMDEPFSALDPTIRRDLQGRFLQLVRERGITTLLVTHDPGEAL
RLADRIAVLRGGRLIQVGTPGELLEHPADEGVAVFFDERPHIGPSIASSSDEVLSEPASA
ELPARSTIHWIRQSVLGPAALAAAGRGGWFWLALLVEVAALAMLAGAWREGAGWLSATAL
AVLLVNRLGCAWVSLHGHGRDSPGVALPLALLLVSQALTVWRALTPEAAGTLVQFPSDRD
SMLAAAGAIDALIGWSQVTFESAFVGVIVAVRTVINGVEGALGWIPWPVPALALVYLAWR
SAGAILALTTALALGYIGLFGFWERTISTIALVGASVLLSLLIGIPTGILLAKRALARRL
VTPLLDVMQTLPTFVYLIPAVAFFSVGKTPAVIATVIFALAPMIRLTALGIQEVPKAAVE
AAVAHGATPWQTLTRVELPLASGSLLLGVNQTIVMSLSMVVVAALIGAGGLGYDVMTALR
NIKGGEGVLAGIAIVFCALVPDRIIQASLRKRHHSTQE