Protein Info for PP_2717 in Pseudomonas putida KT2440

Annotation: arsenite/antimonite transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 47 to 70 (24 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 115 to 160 (46 residues), see Phobius details amino acids 176 to 198 (23 residues), see Phobius details amino acids 222 to 240 (19 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 278 to 296 (19 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details amino acids 401 to 423 (23 residues), see Phobius details PF02040: ArsB" amino acids 4 to 423 (420 residues), 525.5 bits, see alignment E=1.4e-161 TIGR00935: arsenite/antimonite efflux pump membrane protein" amino acids 4 to 427 (424 residues), 688.4 bits, see alignment E=2.3e-211 PF03600: CitMHS" amino acids 12 to 368 (357 residues), 207.6 bits, see alignment E=2.7e-65

Best Hits

Swiss-Prot: 72% identical to ARSB1_ECOLX: Arsenical pump membrane protein (arsB) from Escherichia coli

KEGG orthology group: K03893, arsenical pump membrane protein (inferred from 100% identity to ppu:PP_2717)

MetaCyc: 72% identical to arsenous acid/antimonous acid:H+ antiporter ArsB (Escherichia coli)
RXN-22366 [EC: 7.3.2.7]; 7.3.2.7 [EC: 7.3.2.7]; RXN-22385 [EC: 7.3.2.7]; RXN3O-9786 [EC: 7.3.2.7]

Predicted SEED Role

"Arsenic efflux pump protein" in subsystem Arsenic resistance

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JD2 at UniProt or InterPro

Protein Sequence (427 amino acids)

>PP_2717 arsenite/antimonite transporter (Pseudomonas putida KT2440)
MLPAIAVFVFTLILVIWQPKGLGVGWSAALGAIIALATGVVSLHDIPAVWAIVWNATATF
IAVIIISLLLDEAGFFEWAALHVARWANGSGHRLFAFCVLLGAAVSALFANDGAALILTP
IVMSMLLALRFSPAATLAFVMAAGFIADTASLPLVVSNLVNIVSADYFGLGFAEYASVMV
PVNIASVAATLLVLFLYFRRDIPKQYALDALKAPSEAIHDHATFKVGGWVLLVLLAGLFA
LEPLGIPISAVAAVCAAILFAVAARGHRISTRRVLREAPWQVVIFSLGMYLVVYGLKNAG
LTDMLTHLLDTLAEQGLWSAAIGTGLISALLSSVMNNMPSVLIGALSIQASGAEGLVREA
MIYANVIGCDLGPKITPIGSLATLLWLHVLARKGVRITWGYYFKVGLLLTVPVLLITLSA
LALRLSL