Protein Info for PP_2713 in Pseudomonas putida KT2440

Annotation: DNA-binding response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 220 PF00072: Response_reg" amino acids 4 to 112 (109 residues), 100.7 bits, see alignment E=5.5e-33

Best Hits

Swiss-Prot: 48% identical to QSEB_HAEIN: Transcriptional regulatory protein QseB (qseB) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K02483, two-component system, OmpR family, response regulator (inferred from 100% identity to ppu:PP_2713)

Predicted SEED Role

"Two-component system response regulator QseB" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JD6 at UniProt or InterPro

Protein Sequence (220 amino acids)

>PP_2713 DNA-binding response regulator (Pseudomonas putida KT2440)
MRLLLVEDDIALGEGICDGLRQEGYTLDWLRDGVSGLHALQHETFDLVILDLGLPRMDGL
ELLRRLRAGGDSLPVLILTARDALDDRIAGLDAGADDYLVKPFDLNELKARLRALLRRSV
GRAKVLIEHAGVSLDPTTQQVQYNGVEVVLTPKEYVLLHELLAQPGKVFTRERLTQLLYG
WDEEPESNTLEVNIYHLRKKLFNGLIRTVRGIGYLVEVKA