Protein Info for PP_2709 in Pseudomonas putida KT2440

Annotation: putative Long-chain-fatty-acid--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 transmembrane" amino acids 185 to 212 (28 residues), see Phobius details amino acids 232 to 255 (24 residues), see Phobius details PF00501: AMP-binding" amino acids 13 to 338 (326 residues), 178 bits, see alignment E=2.7e-56 PF23562: AMP-binding_C_3" amino acids 378 to 484 (107 residues), 29.6 bits, see alignment E=8.1e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2709)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JE0 at UniProt or InterPro

Protein Sequence (489 amino acids)

>PP_2709 putative Long-chain-fatty-acid--CoA ligase (Pseudomonas putida KT2440)
MRHELTAFQALLRQHAVEHPDAVAVQGDAQRYTYRQLLNEVESRASCLRSQPPGTFAVVL
DNGPEALFWDLAALFAERASVTVPPFFSATQFQHCLQQSGVTAVVCEAQWAEQLIALGFA
RQAPAGVWQRDSFVAPALPEGTAKITYTSGSTGTPKGVCLSAETLLRVAHELEAASRPTA
PQRYLAVLPLGVLLENLGVYAALMAGACVQLYPQQQLGMGGASQVDFKRLLGAIALSGAQ
SLILVPQLLMGLVTAIERGLMRVGPLRLVAVGGARVSPSLLARAEAVGLPVFEGYGLSEC
ASVVALNRTGAVRPGSVGKPLPHVQVRIAEDGEVLVAGSTLLGYLGEPPVTGQWWATGDV
GHLDEDGYLYLDGRKKHQFITSFGRNVNPEWVEAELSQSGVIAQAFVHGEALPRNLALLW
PLDPGISDEAIEQAVQQCNARLPDYARVHAWRRLPVPLSTLDETLTANGRPRREAILQRY
HTLLSDISL