Protein Info for PP_2645 in Pseudomonas putida KT2440

Annotation: magnesium transporter, ATP-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 920 transmembrane" amino acids 98 to 116 (19 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 310 to 329 (20 residues), see Phobius details amino acids 336 to 361 (26 residues), see Phobius details amino acids 719 to 741 (23 residues), see Phobius details amino acids 747 to 767 (21 residues), see Phobius details amino acids 789 to 812 (24 residues), see Phobius details amino acids 823 to 842 (20 residues), see Phobius details amino acids 854 to 874 (21 residues), see Phobius details amino acids 890 to 912 (23 residues), see Phobius details TIGR01524: magnesium-translocating P-type ATPase" amino acids 32 to 919 (888 residues), 1400.9 bits, see alignment E=0 PF00690: Cation_ATPase_N" amino acids 46 to 109 (64 residues), 50.9 bits, see alignment 2.5e-17 PF00122: E1-E2_ATPase" amino acids 162 to 370 (209 residues), 139.2 bits, see alignment E=2.8e-44 TIGR01494: HAD ATPase, P-type, family IC" amino acids 283 to 428 (146 residues), 81.9 bits, see alignment E=3.5e-27 amino acids 633 to 749 (117 residues), 94.6 bits, see alignment E=4.9e-31 PF00702: Hydrolase" amino acids 386 to 674 (289 residues), 54.9 bits, see alignment E=4.1e-18 PF13246: Cation_ATPase" amino acids 455 to 500 (46 residues), 23.2 bits, see alignment 1.4e-08 PF00689: Cation_ATPase_C" amino acids 743 to 910 (168 residues), 77.6 bits, see alignment E=2.7e-25

Best Hits

KEGG orthology group: K01531, Mg2+-importing ATPase [EC: 3.6.3.2] (inferred from 73% identity to azc:AZC_0192)

Predicted SEED Role

"Mg(2+) transport ATPase, P-type (EC 3.6.3.2)" (EC 3.6.3.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JK4 at UniProt or InterPro

Protein Sequence (920 amino acids)

>PP_2645 magnesium transporter, ATP-dependent (Pseudomonas putida KT2440)
MKRLCVVPPQRQEHSNMTVKYRNTSSAKAERETRLSTRAAREARNGLAVTLANLDASEQG
LTEHEAAKRLVRDGANQVAHDPQPHALVQLLKALNNPFIYVLLTLAGISFVTDYWLPVSA
GEADDADLTKVIIIMTMVSLSSLLRFWQEYRSNKAADALKAMVRTTATVLRREQIGQAPR
LREVPMDELVAGDIVQLSAGDMIPADIRLLEARDLFISQAVLTGEALPVEKYDTLGNVAQ
KSAAEHGAHQDNLLELPNICFMGTNVVSGRARAVVVATGRRTYFGSLAKAIAGSRSQTAF
DRGVNSVSSLLIRFMLVMVPVVFMINGVVKGDWADAFLFALAVAVGLTPEMLPMIVSANL
AKGAVAMARRKVVVKRLNAIQNLGSMDVLCTDKTGTLTQDRIILEHHVGFDGQTDKHILE
LAWLNSHHQSGIRNLMDQAVLHFAGQDHQFQAPYAYAKVDELPFDFIRRRLSVVVKNALG
DHLLVSKGAVEEMLAIATHVQEGDKVVALDPCRRQQLMARVDAFNQDGFRVLVVATRQIP
ADEGKAQYHTEDERDLVIQGLLTFLDPPKETAGPAIAALRDMGVQVKVLTGDNPVVTSKV
CREVGLAPGQPLLGQDIEGMDDTTLKLQVEERTVFAKLTPLQKSRVLKALQANGHTVGFL
GDGINDAAALRDADVGISVDSGTDIAKESADIILLEKSLMVLEEGVLKGRETFGNIMKYL
CMTASSNFGNVFSVLVASAFIPFLPMLAIHLLLQNLMYDFSQLSLPWDRMDKEFLSKPRK
WDARNIGRFMLWIGPTSSIFDITTFALMWYVFAANSVEMQALFQSGWFIEGLLSQTLVVH
MLRTRKVPFFQSTAALPVVLATGLVMALGIYIPFSPVGAMVGLVPLPWEYFPWLVATLLG
YCVVAQAMKTLYIRRFGQWF