Protein Info for PP_2637 in Pseudomonas putida KT2440

Annotation: endo-1,4-D-glucanase subunit of cellulose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF01270: Glyco_hydro_8" amino acids 7 to 348 (342 residues), 259.6 bits, see alignment E=2.1e-81

Best Hits

Swiss-Prot: 52% identical to GUN_XANAC: Endoglucanase (bcsZ) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K01179, endoglucanase [EC: 3.2.1.4] (inferred from 100% identity to ppu:PP_2637)

Predicted SEED Role

"Endoglucanase precursor (EC 3.2.1.4)" (EC 3.2.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JL2 at UniProt or InterPro

Protein Sequence (370 amino acids)

>PP_2637 endo-1,4-D-glucanase subunit of cellulose synthase (Pseudomonas putida KT2440)
MRQMLVGLVALGLPVFANAGSVCPWPAWERFKAELVSVDGRVIDPSDERLITTSEGQSYA
LFFALVGNDRQTFAQLLRWTSNNLAEGDLARHLPAWLWGRDGQQQWQVLDANNASDADLW
IAYSLLEAGRLWDQPAYTQLGQHLLWRIAAQTVRKLPGLGVMLLPGDYGFEDAQGTRLNP
SYLPLQLLDRFSDVDPLWGELAANTRRLWLASSPKGFAPDWLLWTPAGKPAADTKHGNAG
DYDAIRVYLWVGMLAEGAAQRRELVAHYAPMAALTQRQGLPPEHLDARSGEARGHGPAGF
SAALLPLLAASPEHVAGLAAQRQRLREQPVEAKAYYSQVLALFGQGFDEARYRFDPHGRL
LPAWSAPCSE