Protein Info for PP_2626 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR03362: type VI secretion-associated protein, VC_A0119 family" amino acids 13 to 472 (460 residues), 435 bits, see alignment E=2.2e-134 PF06812: ImpA_N" amino acids 16 to 127 (112 residues), 78.9 bits, see alignment E=3.5e-26 PF16989: T6SS_VasJ" amino acids 222 to 471 (250 residues), 242.8 bits, see alignment E=4.4e-76

Best Hits

KEGG orthology group: K11910, type VI secretion system protein VasJ (inferred from 100% identity to ppu:PP_2626)

Predicted SEED Role

"Uncharacterized protein ImpA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JM3 at UniProt or InterPro

Protein Sequence (487 amino acids)

>PP_2626 conserved protein of unknown function (Pseudomonas putida KT2440)
MSVVAQVSGEEVTRLLAPIDPAVPAGLFDVEDETYQAIDQEMVKLGGLQEASIDWVYIED
ASRQYLSQQCKHLRIVAHLSAAWLRSGCWEQWGCTLALLAGMLECYWEAAHPKPGPKGFL
GKRKLVALVIDRLAEALPRLDRFTYTPTHAAAAQAALKRLQQQETVQLDTAALGELERLL
GKYSELANGVGEAAVPKAPAATEKPAPLADVIATPVSRMSMGNERETRRAVLSMAELINQ
QDPYDATGYQLRRFGLWAHIQVAPQVRQGNRTELMAVPLDITRFYEEAIASKAVDPALLQ
RIEKSLTACPFWIRGSFLAATVATQLAMCEVAEAIRCATARFVQRMPSLQQLCFSDGMVF
VDDQCLAWLKGTQGPSGQGATPQEFSGLREELVSQLETGGIEPLLLRLQGLQAGVRAPRE
RCHTTVIAADLLAARGVSWLAQDLCANVARTMQQTTASAWEPEVFERLQQYAASPVLAVQ
DKDQEPR