Protein Info for PP_2584 in Pseudomonas putida KT2440

Annotation: hydroxydechloroatrazine ethylaminohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01979: Amidohydro_1
  • amino acids 63 to 428 (366 residues), 211.7 bits, E=1.8e-66
• PF07969: Amidohydro_3
  • amino acids 183 to 429 (247 residues), 46.8 bits, E=3.3e-16

Best Hits

Swiss-Prot: 44% identical to OXODE_PSEAE: 8-oxoguanine deaminase (PA0142) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03382, hydroxyatrazine ethylaminohydrolase [EC: 3.5.99.3] (inferred from 100% identity to ppf:Pput_3132)

MetaCyc: 44% identical to 8-oxoguanine deaminase monomer (Pseudomonas aeruginosa)
RXN-11455 [EC: 3.5.4.32]

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3)" (EC 3.5.4.3, EC 3.5.99.3)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• superpathway of purines degradation in plants (13/18 steps found)
• drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Atrazine degradation
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3, 3.5.99.3

Use Curated BLAST to search for 3.5.4.3 or 3.5.4.32 or 3.5.99.3

Sequence Analysis Tools

See Q88JR5 at UniProt or InterPro

Protein Sequence (457 amino acids)

>PP_2584 hydroxydechloroatrazine ethylaminohydrolase (Pseudomonas putida KT2440)
MSSTNPAKTLLVKNAALLVTMDGERREIKNGGLFIEGNLIKQVGPSETLPQHADVVLDMA
GKVVIPGLVNTHHHMYQSLTRVVPAAQDGELFNWLTNLYPIWARLTPEMIAVSTQTAMAE
LILSGCTTSSDHLYIYPNGCKLDDSIHAAAEIGMRFHAARGSMSVGQSQGGLPPDSVVEK
EADILKESQRLIEDYHDASHGSMRRIVVAPCSPFSVSRDLMREAAVLARQYGVSLHTHLA
ENVNDIAYSREKFGMTPAEYAEDLGWVGHDVWHAHCVQLDQHGIELFARTGTGVAHCPCS
NMRLASGIAPVRKMRDHGVPVGLGVDGSASNDGASMIGEVRQALLLQRVGFGPDAMTARE
ALEIATLGGAKVLNRNDIGALAPGMVADFVAFDLGHVAYAGGHHDPLAALVFCTPTQVHT
SVINGRVVVKDGQLATVDLPRVLERHNQLAHQLVSGT