Protein Info for PP_2558 in Pseudomonas putida KT2440

Annotation: putative Outer membrane efflux protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 29 to 444 (416 residues), 311.4 bits, see alignment E=5e-97 PF02321: OEP" amino acids 33 to 220 (188 residues), 86.1 bits, see alignment E=1.4e-28 amino acids 243 to 429 (187 residues), 106.1 bits, see alignment E=1e-34

Best Hits

KEGG orthology group: K12538, outer membrane protein HasF (inferred from 100% identity to ppu:PP_2558)

Predicted SEED Role

"ABC-type protease exporter, outer membrane component PrtF/AprF" in subsystem Protein secretion by ABC-type exporters

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JT9 at UniProt or InterPro

Protein Sequence (452 amino acids)

>PP_2558 putative Outer membrane efflux protein (Pseudomonas putida KT2440)
MWTPRSRSNDMKRPCLMVCLLGVAVQASAMDLKQAWDLLQYQGPIYRAAVHEKQAGGENR
AIGEAGLLPQINASAYFNQVNGSQRQNGQDNDLDYDSKGANVRLRQPLFNKQKMAEYRQG
QQRADYSVAVFDAKSQDAAVRLADAYFDVLLASETITLAKAKLSAFEEQLASAKRRMELG
AGTITDVDESVARRDLAEADLIDAQDNLVNARRKLEEYIGETPQALTTLQAGFSTPPLMP
GNLQEWLVKAQADSPLIHARRHSYALAEEEVNRARSGHYPTLDFVAGYTAGSSQSISELN
QRNHYSSIGLELNIPLYSGGSTSALSRQASANSSKALDELDATRQEVISNTTREYRGVQS
GALRIQALEKAVASNERSLTSTRKGFKEGGTSTNSDVLNAEELLYDARHDLFEAKLNYLM
SRLRLASSVGSLGDDDIEQINDYLGPELIVSN