Protein Info for PP_2553 in Pseudomonas putida KT2440

Annotation: Major facilitator family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 49 to 72 (24 residues), see Phobius details amino acids 84 to 102 (19 residues), see Phobius details amino acids 108 to 128 (21 residues), see Phobius details amino acids 149 to 173 (25 residues), see Phobius details amino acids 184 to 203 (20 residues), see Phobius details amino acids 239 to 263 (25 residues), see Phobius details amino acids 275 to 295 (21 residues), see Phobius details amino acids 307 to 326 (20 residues), see Phobius details amino acids 332 to 352 (21 residues), see Phobius details amino acids 368 to 391 (24 residues), see Phobius details amino acids 398 to 420 (23 residues), see Phobius details PF00083: Sugar_tr" amino acids 17 to 418 (402 residues), 91.6 bits, see alignment E=8e-30 PF07690: MFS_1" amino acids 17 to 377 (361 residues), 111.1 bits, see alignment E=8.8e-36 PF13347: MFS_2" amino acids 79 to 360 (282 residues), 37 bits, see alignment E=2.3e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2553)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JU4 at UniProt or InterPro

Protein Sequence (457 amino acids)

>PP_2553 Major facilitator family transporter (Pseudomonas putida KT2440)
MAHSSSQAKKATASGWIGSALEYYDFFIYAQAAALIFPQIFFPNTDPKMAIIASLATYGV
GYLARPIGAFVLGHWGDTRGRKNVLLLCMFLMGLSTMAVGLLPTYHDIGYLAPALLVVLR
LIQGFAVAGEISGASSMIMEHAPFGRRGYYASFTLQGVQAGQVLAAAVFLPLAYFMPSEA
FTEWGWRIPFLMSAIVLIAGFIIRKEVHETPAFVQEEKQDKVAKSPISEAFRHSWKHMVL
VMFMALMNVIPVVATIFGAAYAVQPAYGIGFDKSVYLWIPVVGNIVAVLVIPFVGNLSDK
IGRRPTMIAGCLGSGLLAFVYLYAISIQNVPMAFAASIVMWGMVYQGYNAVFPSFYPELF
HTRYRVTAMAIAQNIGTMLTAMLPALFAMVAPPGSDNIPLVVGGLAFFITGVCALAAYIA
PETHRLAMEDLGNPDAKPMEKAAYEASRKGSFQAVSH