Protein Info for PP_2545 in Pseudomonas putida KT2440

Annotation: trans-aconitate methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 PF13489: Methyltransf_23" amino acids 28 to 164 (137 residues), 36.3 bits, see alignment E=1.6e-12 PF05175: MTS" amino acids 36 to 117 (82 residues), 24.6 bits, see alignment E=6e-09 PF01728: FtsJ" amino acids 44 to 102 (59 residues), 24 bits, see alignment E=1.1e-08 PF13847: Methyltransf_31" amino acids 49 to 155 (107 residues), 45.1 bits, see alignment E=3.3e-15 PF08241: Methyltransf_11" amino acids 50 to 144 (95 residues), 42.8 bits, see alignment E=2.5e-14 PF13649: Methyltransf_25" amino acids 50 to 136 (87 residues), 51.5 bits, see alignment E=4.9e-17 PF08242: Methyltransf_12" amino acids 50 to 141 (92 residues), 40.7 bits, see alignment E=1.1e-13

Best Hits

Swiss-Prot: 49% identical to TAM_RHILO: Trans-aconitate 2-methyltransferase (tam) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K00598, trans-aconitate 2-methyltransferase [EC: 2.1.1.144] (inferred from 100% identity to ppu:PP_2545)

MetaCyc: 44% identical to trans-aconitate 2-methyltransferase (Escherichia coli K-12 substr. MG1655)
Trans-aconitate 2-methyltransferase. [EC: 2.1.1.144]

Predicted SEED Role

"Trans-aconitate 2-methyltransferase (EC 2.1.1.144)" (EC 2.1.1.144)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.144

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JV2 at UniProt or InterPro

Protein Sequence (272 amino acids)

>PP_2545 trans-aconitate methyltransferase (Pseudomonas putida KT2440)
MAYARHIVRPKEPHMAWSANQYSLFEDERTRAVRDLIAAIPPRPVRHATDLGCGPGNSTE
VLLQRYPDAQVTALDSDPDMIDKARERKRLCIPRVRTVIADIAGWTAPEPQDLILANASL
QWVPDHALLYPHLVRQLSEGASLAVQTPDNLDEPAHRQLREIASQGPWAAKFADLQLPPR
HNAAFYYDLLSPLCARVDVWRTTYHHPLVGGAEAVVEWFKGSALRPYLARLDEQEQADFL
QMYLQAMQRDYPPASDGKVLLPFPRLFVIATR