Protein Info for PP_2535 in Pseudomonas putida KT2440

Annotation: putative inner membrane transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 38 to 60 (23 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 97 to 122 (26 residues), see Phobius details amino acids 129 to 149 (21 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 203 to 225 (23 residues), see Phobius details amino acids 237 to 256 (20 residues), see Phobius details amino acids 268 to 287 (20 residues), see Phobius details amino acids 293 to 312 (20 residues), see Phobius details amino acids 325 to 349 (25 residues), see Phobius details amino acids 355 to 378 (24 residues), see Phobius details PF07690: MFS_1" amino acids 10 to 322 (313 residues), 133.3 bits, see alignment E=1.5e-42 PF06779: MFS_4" amino acids 15 to 373 (359 residues), 35.4 bits, see alignment E=1.2e-12 PF00083: Sugar_tr" amino acids 42 to 167 (126 residues), 27.6 bits, see alignment E=2.2e-10

Best Hits

Swiss-Prot: 42% identical to YFHI_BACSU: Uncharacterized MFS-type transporter YfhI (yfhI) from Bacillus subtilis (strain 168)

KEGG orthology group: K08156, MFS transporter, DHA1 family, arabinose polymer transporter (inferred from 100% identity to ppu:PP_2535)

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88JW2 at UniProt or InterPro

Protein Sequence (391 amino acids)

>PP_2535 putative inner membrane transport protein (Pseudomonas putida KT2440)
MRINPPLVALAIGAFGIGVTEFAPMGMLPSIATDLGVSIPAAGLLVSAYAIGVLIGAPLM
TLATVKIPRRYLLIGLMAIFTLGNLMSALASDYASLLVARVVTSLNHGAFFGIGSIVAAS
VVPPEKRAGAVAAMFMGLTLATIGGVPLATWLGEMLGWRTAFWGIAGLGLVAMTTLWYAL
PNVASPKGEGAMAEIRVLGRRPVLAALLLTVVGSSAMFTVFTYIAPILQHEAAASTPFVT
AMLVLFGVGLTLGNVWGGKAADRSIDRTLILSLAGLIAVLLVFPLVLDWPLPTALAILVW
GAASFALVPPLQMRVMEAAKDAPNLASAVNIGAFNLGNAIGAALGGAVINAGLGYPAISL
AGAAMAALGLVLVLLFAWRGRGVVAGGQVAL