Protein Info for PP_2453 in Pseudomonas putida KT2440

Annotation: Glutaminase-asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to ASPQ_PSEPK: Glutaminase-asparaginase (ansB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K05597, glutamin-(asparagin-)ase [EC: 3.5.1.38] (inferred from 100% identity to ppf:Pput_3237)

MetaCyc: 47% identical to L-asparaginase 2 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

L-citrulline biosynthesis (7/8 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
L-glutamate and L-glutamine biosynthesis (6/7 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
superpathway of L-citrulline metabolism (9/12 steps found)
L-asparagine degradation I (1/1 steps found)
L-glutamine degradation I (1/1 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1

Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88K39 at UniProt or InterPro

Protein Sequence (362 amino acids)

>PP_2453 Glutaminase-asparaginase (Pseudomonas putida KT2440)
MNAALKTFAPSALALLLILPSSASAKEAETQQKLANVVILATGGTIAGAGASAANSATYQ
AAKLGVDKLIAGVPELADIANVRGEQVMQIASESISNDDLLKLGKRVAELAESKDVDGIV
ITHGTDTLEETAFFLNLVEKTDKPIVVVGSMRPGTAMSADGMLNLYNAVAVASDKQSRGK
GVLVTMNDEIQSGRDVSKAVNIKTEAFKSAWGPMGMVVEGKSYWFRLPAKRHTVNSEFDI
KQISSLPQVDIAYGYGNVTDTAYKALAQNGAKALIHAGTGNGSVSSRVVPALQELRKNGV
QIIRSSHVNQGGFVLRNAEQPDDKNDWVVAHDLNPQKARILAMVAMTKTQDSKELQRIFW
EY