Protein Info for PP_2445 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 54 to 74 (21 residues), see Phobius details amino acids 86 to 103 (18 residues), see Phobius details amino acids 126 to 148 (23 residues), see Phobius details amino acids 161 to 179 (19 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 220 to 238 (19 residues), see Phobius details PF03741: TerC" amino acids 19 to 210 (192 residues), 166.2 bits, see alignment E=3.2e-53

Best Hits

Swiss-Prot: 53% identical to YGDQ_ECO57: UPF0053 inner membrane protein YgdQ (ygdQ) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 98% identity to ppg:PputGB1_3527)

Predicted SEED Role

"Integral membrane protein TerC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88K47 at UniProt or InterPro

Protein Sequence (250 amino acids)

>PP_2445 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MEYLLELAASPTAWVALATLVAMEVVLGIDNLIFISILTNKLPVEYRSKARRIGISMALV
MRLALLSTVAWIVQLTDPVFEVFGNAFSWKDVILIAGGLFLLWKATKEIHENVDPHGAKE
EAKVSSTVTLGFAAAIFQILLLDIVFSVDSIITAVGMTEHLPIMIIAVITAVIVMMVAAD
PLANFINDNPTVVMLALGFLIMIGMTLIAEGFGAHVPKGYVYAAMAFSTAIEVLNILARR
ARLKREAAEG