Protein Info for PP_2428 in Pseudomonas putida KT2440

Annotation: Probable sugar efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 transmembrane" amino acids 26 to 46 (21 residues), see Phobius details amino acids 63 to 86 (24 residues), see Phobius details amino acids 95 to 113 (19 residues), see Phobius details amino acids 120 to 142 (23 residues), see Phobius details amino acids 154 to 173 (20 residues), see Phobius details amino acids 181 to 203 (23 residues), see Phobius details amino acids 225 to 246 (22 residues), see Phobius details amino acids 263 to 280 (18 residues), see Phobius details amino acids 289 to 307 (19 residues), see Phobius details amino acids 313 to 334 (22 residues), see Phobius details amino acids 348 to 371 (24 residues), see Phobius details amino acids 378 to 398 (21 residues), see Phobius details PF07690: MFS_1" amino acids 33 to 364 (332 residues), 125.2 bits, see alignment E=1.5e-40

Best Hits

Swiss-Prot: 100% identical to SOTB_PSEPK: Probable sugar efflux transporter (sotB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03445, MFS transporter, DHA1 family, purine ribonucleoside efflux pump (inferred from 100% identity to ppu:PP_2428)

MetaCyc: 58% identical to L-arabinose exporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-40

Predicted SEED Role

"Sugar efflux transporter SotB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88K63 at UniProt or InterPro

Protein Sequence (408 amino acids)

>PP_2428 Probable sugar efflux transporter (Pseudomonas putida KT2440)
MSAEKRFPPMNGPIPTHLPPTTGSGSWLSVIALALAAFIFNTTEFVPVALLSDIGRSFDM
STAQVGLMLTIYAWIVALASLPMMLLTRNIERRRLLLFVFLVFIVSHLLSWLSQSFAMLL
VSRIGIALAHAVFWSITASLAVRVAPPGQQAKALGLLATGTTLAMVLGIPLGRVVGEALG
WRITFLSIAGVALATMLCLIKSLPLLPSQNSGSLRSLPVLFKRPALVITYLLVTLVITAQ
FTAYSYIEPFALHVAQIGGDRTTLLLLLFGGAGVFGSLLFSRYSERFPHGFLVGSIGVLA
ACLLLMLPLSGNFYVFAVLSMFWGVAILSFSLALQSKTLKLASDATDVAMALFSGIYNIG
IGGGALLGSIVSSQMDVADIGLVGGSVAVVGLVLAVASTRRFREALTR