Protein Info for PP_2375 in Pseudomonas putida KT2440

Annotation: cobalamin-dependent methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 15 to 1201 (1187 residues), 1766.7 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 20 to 328 (309 residues), 324.2 bits, E=2.8e-100
• PF00809: Pterin_bind
  • amino acids 363 to 601 (239 residues), 209.6 bits, E=1.5e-65
• PF02607: B12-binding_2
  • amino acids 661 to 733 (73 residues), 83 bits, 3.6e-27
• PF02310: B12-binding
  • amino acids 749 to 842 (94 residues), 70.8 bits, 2.4e-23
• PF02965: Met_synt_B12
  • amino acids 941 to 1217 (277 residues), 405.6 bits, E=2.8e-125

Best Hits

Swiss-Prot: 88% identical to METH_PSEAE: Methionine synthase (metH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 68% identity to aci:ACIAD1045)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine salvage from L-homocysteine (2/3 steps found)
• folate transformations I (9/13 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See Q88KB5 at UniProt or InterPro

Protein Sequence (1235 amino acids)

>PP_2375 cobalamin-dependent methionine synthase (Pseudomonas putida KT2440)
MSDRSARLQALQNALKERILILDGGMGTMIQSYRLEEHDYRGTRFADWPSDVKGNNDLLL
LSRPDVIAAIEKAYLDAGADILETNTFNATQISQADYGMESLVYELNVEGARIARQVADA
KTLETPDKPRFVAGVLGPTSRTCSISPDVNDPGFRNVTFDELVENYIEATRGLIEGGADL
ILIETIFDTLNAKAAIFAVQQVFEDDNVELPIMISGTITDASGRTLSGQTTEAFWNSVRH
AKPISVGLNCALGAKDLRPYLEELATKADTHVSAHPNAGLPNAFGEYDETPAEMAAVVEE
FAASGFLNIIGGCCGTTPGHIQAIAEAVAKYKPREIPEIAKACRLSGLEPFTIDRQSLFV
NVGERTNITGSAKFARLIREENYTEALEVALQQVEAGAQVIDINMDEGMLDSQAAMVRFL
NLIAGEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEQFKHHARLCKRYG
AAVVVMAFDEVGQADTAARKKEICQRSYDILVNEVGFPPEDIIFDPNIFAVATGIEEHNN
YAVDFIEACAYIRDHLPHALSSGGVSNVSFSFRGNNPVREAIHSVFLYHAIQNGLTMGIV
NAGQLEIYDEIPAQLREKVEDVVLNRTPHGTDALLAIADDYKGGGATKEVENEEWRSLPV
EKRLEHALVKGITAFIVEDTEACRQQCARPIEVIEGPLMNGMNVVGDLFGAGKMFLPQVV
KSARVMKQAVAHLIPFIEAEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIV
DLGVMVPAEKILQTAREQKCDIIGLSGLITPSLDEMVHVAREMQRQGFELPLMIGGATTS
KAHTAVKIEPKYSNDAVIYVTDASRAVGVATQLLSKELKPGFVEKTRLEYVDVRERTANR
SARTERLSYAQAIAAKPQYDWAGYQPTAPSFTGVKVLEDIDLRTLAEYIDWTPFFISWDL
AGKFPRILTDEVVGEAATALYKDAREMLDKLIDEKLISARAVFGFWPANQVDDDDIEVYG
EDGQALATLHHLRQQTIKPDGKPNWSLADFVAPKDSGVTDYVGGFITTAGIGAEEVAKAY
QDKGDDYSSIMVKALADRLAEACAEWLHEQVRKEHWGYARDEHLDNEALIKEQYSGIRPA
PGYPACPDHTEKETLFRLLDGTAIGETGPSGVYLTEHFAMFPAAAVSGWYFAHPQAKYFA
VGKVDKDQIERYSARKGQDISVSERWLAPNLGYDS