Protein Info for PP_2304 in Pseudomonas putida KT2440

Annotation: putative Peptidyl-prolyl cis-trans isomerase D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 623 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details PF13624: SurA_N_3" amino acids 1 to 164 (164 residues), 152.3 bits, see alignment E=2.6e-48 PF13623: SurA_N_2" amino acids 2 to 140 (139 residues), 112.1 bits, see alignment E=5.2e-36 PF13145: Rotamase_2" amino acids 245 to 375 (131 residues), 60.8 bits, see alignment E=5.9e-20 PF13616: Rotamase_3" amino acids 261 to 360 (100 residues), 101.2 bits, see alignment E=1.4e-32 PF00639: Rotamase" amino acids 269 to 359 (91 residues), 97.3 bits, see alignment E=2.2e-31

Best Hits

KEGG orthology group: K03770, peptidyl-prolyl cis-trans isomerase D [EC: 5.2.1.8] (inferred from 100% identity to ppu:PP_2304)

Predicted SEED Role

"Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8)" (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KI6 at UniProt or InterPro

Protein Sequence (623 amino acids)

>PP_2304 putative Peptidyl-prolyl cis-trans isomerase D (Pseudomonas putida KT2440)
MLQNIRDNSQGWIAKTIIGLIVVLMALTGFEAIFQAATHSQDAAKVNGQTISQNELSQAA
DMQRRQLMQQLGKDFDPALLDDKLLREEALKGLISRKLLLQGAEDAKFAFSEAALDQVIL
QTPEFQVDGKFSAERFDQVIRQMGYGRMQFREMLGEEMLIGQLRTGLAGSSFVTDQQVDA
FARLEKQTRDFASLTFKADPAAVKVSDEEVKAHYDQHAKEFMSPDQVVIDYIELKKSAFF
DQVKVTDEELKAQYEKEIANLAEQRHAAHILIEVNDKVTDAQAKARAEEIEQRLAKGEDF
AALAKEFSQDPGSANTGGDLGFAGPGVYDPAFEEALYKLQDGQVSAPVRTEFGYHLIKLL
GVQAPEVPSFASLKDKLTRDLKMPLVEQRYVDASKQLQDAAYEASDLAQPAQDLNLKVHT
SAAFGREGGEGITANRAVVQAAFSEEVLDEGANSTAIELDPETTVVLRVKEHRKPEQLPL
DAVAKNIREHLAKEKATAELKAKADKLIGGLRDGSIAAGSVHEGQGWKAYEAVTRGEDGI
DPAELQALFRLGKPQAKDKPVYGSVVLRDGSLVVLQLKGVNEGAAATDEEKQQIRRYLAS
RAGQQDFAAYRKQLEANADITRY