Protein Info for PP_2265 in Pseudomonas putida KT2440

Annotation: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 121 (116 residues), 141.8 bits, E=1.1e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 124 to 281 (158 residues), 224.9 bits, E=3.3e-71

Best Hits

Swiss-Prot: 100% identical to FOLD1_PSEPG: Bifunctional protein FolD 1 (folD1) from Pseudomonas putida (strain GB-1)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 100% identity to ppg:PputGB1_1899)

MetaCyc: 66% identical to bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase (Escherichia coli K-12 substr. MG1655)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• folate transformations I (9/13 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.5, 3.5.4.9

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See Q88KM5 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PP_2265 methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (Pseudomonas putida KT2440)
MTAHLIDGKAIAASLRQQIAQRVVERRQQGLRTPGLAVILVGTDPASQVYVSHKRKDCEE
VGFISQAFDLPSETTQQALTELIDRLNDDPAVDGILLQLPLPAHLDASLLLERIRPDKDV
DGFHPYNIGRLAQRIPLLRPCTPKGIMTLLESTGQDLYGMNAVIVGASNIVGRPMAMELL
LAGCTVTVCHRFTKDLAGHVGRADLVVVAAGKPGLVKGEWVKEGAIVIDVGINRQEDGKL
VGDVVYETALPRAGWITPVPGGVGPMTRACLLENTLYAAEELHK