Protein Info for PP_2240 in Pseudomonas putida KT2440

Annotation: putative ABC efflux transporter, permease/ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 626 transmembrane" amino acids 37 to 60 (24 residues), see Phobius details amino acids 85 to 108 (24 residues), see Phobius details amino acids 123 to 148 (26 residues), see Phobius details amino acids 203 to 226 (24 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details amino acids 304 to 320 (17 residues), see Phobius details amino acids 327 to 345 (19 residues), see Phobius details PF06472: ABC_membrane_2" amino acids 79 to 349 (271 residues), 216.2 bits, see alignment E=8.5e-68 PF05992: SbmA_BacA" amino acids 89 to 395 (307 residues), 90.5 bits, see alignment E=2.3e-29 PF00005: ABC_tran" amino acids 435 to 568 (134 residues), 70.4 bits, see alignment E=3.6e-23

Best Hits

KEGG orthology group: K02471, putative ATP-binding cassette transporter (inferred from 100% identity to ppu:PP_2240)

Predicted SEED Role

"Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KQ0 at UniProt or InterPro

Protein Sequence (626 amino acids)

>PP_2240 putative ABC efflux transporter, permease/ATP-binding protein (Pseudomonas putida KT2440)
MRHLYLTNDSVMLRSRKYSARILTMEMNWHQALQESLSWLAIASFITLVSFTAAATLAVR
FTRWGSQFWQLAGPYFSFRRSWRPLLVFALLLVLTLFSVRLNVLFSFWYNGFYSALQGLD
QAAFWYLLGVFAVLATIHVLRSLFTFYVSQAFSIKWRVWLTERLTHDWMQGDAYYRGQFL
AEPVDNPDQRIELDVNAFVTNSVSLALGAVSALVSLVAFTGILWGLSAPLTVAGVEIPRA
MVFAVYLYVIIATWVAFRLGRPLIRLNFLNEKLTANFRYALMRLRENAENIAFYQGAQVE
RGTLLGRFAALIVNVWALVYRNLKFSGFNLGVSQVAVVFPFILQAPRFFSGAIKLGDVMQ
TSQAFGQVQDSLSFFRESYDAFAQYRATLDRLTGFLDANQQASALPRVTTEDQAHALQIT
GLQVLRPDGHALIADLDLSLHAGQALLIKGPSGSGKTTLLRALAGLWPYAEGKVRRPTGH
QALFLSQRPYLPLGDLRTVIAYPAASNPKDELLMQQALRQVNLAHLAERLDVSCDWSHIL
SVGEQQRLAFARVLFNRPQVVFLDESTSAMDEGLEHALYALLRSEMPSALLVSVGHRSTL
AAFHTHRLEVDGHGGWSLLEQEAVLA