Protein Info for PP_2228 in Pseudomonas putida KT2440

Annotation: putative K(+)/H(+) antiporter subunit D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 37 to 55 (19 residues), see Phobius details amino acids 76 to 103 (28 residues), see Phobius details amino acids 111 to 130 (20 residues), see Phobius details amino acids 136 to 154 (19 residues), see Phobius details amino acids 166 to 189 (24 residues), see Phobius details amino acids 212 to 236 (25 residues), see Phobius details amino acids 245 to 267 (23 residues), see Phobius details amino acids 285 to 304 (20 residues), see Phobius details amino acids 311 to 331 (21 residues), see Phobius details amino acids 337 to 356 (20 residues), see Phobius details amino acids 404 to 425 (22 residues), see Phobius details amino acids 456 to 477 (22 residues), see Phobius details amino acids 498 to 516 (19 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 134 to 442 (309 residues), 95.7 bits, see alignment E=1.6e-31

Best Hits

KEGG orthology group: K05561, multicomponent K+:H+ antiporter subunit D (inferred from 100% identity to ppu:PP_2228)

Predicted SEED Role

"Na(+) H(+) antiporter subunit D" in subsystem Sodium Hydrogen Antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KR2 at UniProt or InterPro

Protein Sequence (559 amino acids)

>PP_2228 putative K(+)/H(+) antiporter subunit D (Pseudomonas putida KT2440)
MSDLSQLIVAPILLPLITAAIMLLLGEKHRHIKARLNLLSTFSGLAIAACLLLWVRTQGQ
AASIGVYLPGNWPAPFGIVLVVDHLSALLLTLTGVIGFSALLFARARWDGAGASFHALFQ
IQLMGLYGAFLTADLFNLFVFFEVLLAASYGLLLHGSGRARVRAGLHYIAINLFASSLFL
IGAAMLYGVTGTLNMADLALKIPLVPEADRGLLHAGAAILAMAFLVKAGIWPLNFWLVPA
YASASAPVAALFAIMTKVGLYAVLRLWTLLFSGQAGASAHFGGEWLVYGGLVTLAVAAVS
ILAAQRLERMAALSILVSAGTLLGAVGFAQAALTGAALFYLVNSTLALCALFLLAELVER
SRSANEAPLDEEEDAMPPPLESLHPPKGINLDDEQQVVIGQIIPWTMAFLGLSFIACALL
IIGMPPLSGFVGKLNLISALFNPLGLGVPAEQPLSAAGWGLVALLILSGLASLIAFGRVG
IQRFWKPEERPSPVLRRYECLPIVILLGLCLILSFKAEPLLRYTQDTAASLQAPDAYIKA
VMAAQPIPGPTSRDVQVQP