Protein Info for PP_2227 in Pseudomonas putida KT2440

Annotation: putative Multicomponent potassium-proton antiporter, subunit E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 162 transmembrane" amino acids 7 to 24 (18 residues), see Phobius details amino acids 30 to 47 (18 residues), see Phobius details amino acids 59 to 82 (24 residues), see Phobius details amino acids 107 to 123 (17 residues), see Phobius details PF01899: MNHE" amino acids 12 to 160 (149 residues), 132.5 bits, see alignment E=5.3e-43

Best Hits

Swiss-Prot: 38% identical to PHAE_RHIME: Probable K(+)/H(+) antiporter subunit E (phaE) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K05562, multicomponent K+:H+ antiporter subunit E (inferred from 100% identity to ppu:PP_2227)

Predicted SEED Role

"Na(+) H(+) antiporter subunit E" in subsystem Sodium Hydrogen Antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KR3 at UniProt or InterPro

Protein Sequence (162 amino acids)

>PP_2227 putative Multicomponent potassium-proton antiporter, subunit E (Pseudomonas putida KT2440)
MNRLFPAPLLSVSLFGLWLLLNLSVSPGNLLLGAALGILAPLLMAPLRPQHAHVRRPLAI
ARLICRVGIDVITSNLLVARGVLRADKQPPRSAFVHIPLALRDPHGLAALSMITTVVPGT
VWSELALDRSVLLLHVFELDDEAAFIQHFKDTYERPLMEIFQ