Protein Info for PP_2151 in Pseudomonas putida KT2440

Annotation: soluble pyridine nucleotide transhydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 transmembrane" amino acids 180 to 199 (20 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 6 to 325 (320 residues), 242.1 bits, see alignment E=4.8e-75 PF01134: GIDA" amino acids 7 to 74 (68 residues), 22 bits, see alignment E=4.5e-08 PF00890: FAD_binding_2" amino acids 7 to 45 (39 residues), 22.9 bits, see alignment 2.5e-08 PF12831: FAD_oxidored" amino acids 7 to 74 (68 residues), 41.3 bits, see alignment E=7.2e-14 PF13450: NAD_binding_8" amino acids 10 to 68 (59 residues), 24.6 bits, see alignment E=1.4e-08 PF13738: Pyr_redox_3" amino acids 134 to 309 (176 residues), 34.9 bits, see alignment E=5.6e-12 PF00070: Pyr_redox" amino acids 177 to 250 (74 residues), 59 bits, see alignment E=3.1e-19 PF02852: Pyr_redox_dim" amino acids 344 to 455 (112 residues), 88.8 bits, see alignment E=1.6e-28

Best Hits

Swiss-Prot: 100% identical to STHA_PSEP1: Soluble pyridine nucleotide transhydrogenase (sthA) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K00322, NAD(P) transhydrogenase [EC: 1.6.1.1] (inferred from 100% identity to ppu:PP_2151)

MetaCyc: 60% identical to soluble pyridine nucleotide transhydrogenase (Escherichia coli K-12 substr. MG1655)
NAD(P)H oxidase. [EC: 1.6.3.1]; 1.6.3.- [EC: 1.6.3.1]; NAD(P)(+) transhydrogenase (B-specific). [EC: 1.6.3.1, 1.6.1.1, 1.6.1.3]

Predicted SEED Role

"Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1)" in subsystem Phosphate metabolism (EC 1.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.1.1 or 1.6.1.3 or 1.6.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88KY8 at UniProt or InterPro

Protein Sequence (464 amino acids)

>PP_2151 soluble pyridine nucleotide transhydrogenase (Pseudomonas putida KT2440)
MAVYNYDVVVLGSGPAGEGAAMNAAKAGRKVAMVDDRRQVGGNCTHLGTIPSKALRHSVR
QIMQFNTNPMFRAIGEPRWFSFPDVLKSAEKVIAKQVASRTGYYARNRVDVFVGTGSFAD
EQTVEVVCPNGVVEKLNAKHIIIATGSRPYRPADIDFHHPRVYDSDTILSLSHTPRKLIV
YGAGVIGCEYASIFSGLGVLVELVDNRGQLLSFLDSEISQALSYHFSNNNITVRHNEEYE
RVEGLDNGVILHLKSGKKIKADALLWCNGRTGNTDKLGLENIGIKVNSRGQIEVDEAYRT
TVPNIYGAGDVIGWPSLASAAHDQGRSAAGSIVDNGSWRFVNDVPTGIYTIPEISSIGKN
EQELTQAKVPYEVGKAFFKSMARAQIAGEPQGMLKILFHRETLEILGVHCFGYQASEIVH
IGQAIMNQPGEQNNLKYFVNTTFNYPTMAEAYRVAAYDGLNRLF