Protein Info for PP_2125 in Pseudomonas putida KT2440

Annotation: lipid kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 TIGR03702: lipid kinase YegS" amino acids 19 to 305 (287 residues), 467.2 bits, see alignment E=1.8e-144 PF00781: DAGK_cat" amino acids 21 to 138 (118 residues), 89.8 bits, see alignment E=1e-29 TIGR00147: lipid kinase, YegS/Rv2252/BmrU family" amino acids 22 to 300 (279 residues), 189.2 bits, see alignment E=8.1e-60 PF19279: YegS_C" amino acids 155 to 296 (142 residues), 48.5 bits, see alignment E=8.5e-17

Best Hits

Swiss-Prot: 100% identical to YEGS_PSEPK: Probable lipid kinase YegS-like (PP_2125) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K07029, (no description) (inferred from 100% identity to ppu:PP_2125)

Predicted SEED Role

"Transcription regulator [contains diacylglycerol kinase catalytic domain]"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L12 at UniProt or InterPro

Protein Sequence (309 amino acids)

>PP_2125 lipid kinase (Pseudomonas putida KT2440)
MVEIGGGPFYGDAHMQGRKAMLVLHGKQAMNEDVRSAVGDLRDSGWVLDVRVTWEAGDAQ
RLVAEALAAGYSHIVAGGGDGTLRDVAEAMGLAATQASLALLPLGTANDFAKAAGIPLEP
ASALALLNVAPQPIDLGQAGDQLFLNMATGGFGSQVTANTSEDLKKVLGAAAYLFTGLSR
FSELQAASVELQGPGFHWQGDLLALGIGNGRQAGGGQVLCPEAMVNDGLLDVAILPAPQE
VVGALRDLLGGDGLFVRARLPWVEIKSSQGLDINLDGEPLQAANLRFQARPAALHLHLPA
GSPLLSHPG