Protein Info for PP_2095 in Pseudomonas putida KT2440

Annotation: Dihydroorotate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to PYRD_PSEPK: Dihydroorotate dehydrogenase (quinone) (pyrD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 100% identity to ppu:PP_2095)

MetaCyc: 60% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
UMP biosynthesis I (6/6 steps found)
UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L40 at UniProt or InterPro

Protein Sequence (340 amino acids)

>PP_2095 Dihydroorotate dehydrogenase (quinone) (Pseudomonas putida KT2440)
MYTLARQLLFKLSPETSHDLSLDLIGAGGRLGLNGMLCKQPASLPVSVMGLNFANPVGLA
AGLDKNGAAIDGFAQLGFGFVEIGTVTPRPQPGNPKPRLFRLPEATAIINRMGFNNLGVD
HLLGRVRASRYKGVLGINIGKNFDTPVERAVDDYLICLDKVYTAASYITVNVSSPNTPGL
RSLQFGDSLKQLLDALAERREQLAGAHGKRVPLAIKIAPDMSDEETALVAAALMESGMDA
VIATNTTLGREGVEALPYGGEAGGLSGAPVLEKSTHIVKVLAGELGGKLPIIAAGGITEG
RHAAEKIAAGASLVQIYSGFIYKGPALIREAVDAIAAMPR