Protein Info for PP_2068 in Pseudomonas putida KT2440

Annotation: putative Multidrug efflux MFS membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 transmembrane" amino acids 69 to 90 (22 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 109 to 155 (47 residues), 41.8 bits, see alignment 1.1e-14 PF16576: HlyD_D23" amino acids 232 to 364 (133 residues), 56.5 bits, see alignment E=3.8e-19 PF13437: HlyD_3" amino acids 288 to 398 (111 residues), 55 bits, see alignment E=1.9e-18

Best Hits

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 100% identity to ppu:PP_2068)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L67 at UniProt or InterPro

Protein Sequence (425 amino acids)

>PP_2068 putative Multidrug efflux MFS membrane fusion protein (Pseudomonas putida KT2440)
MDGTGFARVRGQARSHTRQLLEIEQDSGSHNRQPAGPIRDWKPTMNVAVDKPSPAETEQR
PSAKTRRRLAVIASGSLAAITLLAFTSYWFSTGRYLETTDDAYVRADWVALSPRVAGYVA
KVEVADDQPVKAGDVLVRLQNRDYRARLDQARAGVTEAQAALAAAQASQQVATERIDQQQ
QAILQAEAVVRSATAEQRRSELDVQRYRGLVRDDAATVQRLETASAHASQAQAALQGAQA
ALRQQRSQLAMAKARAAQAEAELQQRAAALARAQAHQQLAEQDEQDTVIRAPITGVVGQR
RVRTGQYVVPGQPLLAVVPLQQAYVVANYKETQLARMRPGQPVEIRVDSFASQPLHGHVA
SFSPASGNVFALLPSDNATGNFTKIVQRFPVRILLDKPLDGPQVLPGMSVVSTVDTRPAE
VADAH