Protein Info for PP_2064 in Pseudomonas putida KT2440

Annotation: Multidrug efflux RND membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 41 to 350 (310 residues), 238.9 bits, see alignment E=3.6e-75 PF13533: Biotin_lipoyl_2" amino acids 65 to 113 (49 residues), 27.6 bits, see alignment 2.9e-10 PF16576: HlyD_D23" amino acids 65 to 265 (201 residues), 89.3 bits, see alignment E=3.4e-29 PF13437: HlyD_3" amino acids 163 to 262 (100 residues), 38.4 bits, see alignment E=2.8e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_3679)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L71 at UniProt or InterPro

Protein Sequence (365 amino acids)

>PP_2064 Multidrug efflux RND membrane fusion protein (Pseudomonas putida KT2440)
MNKRTLLAWAGAVAGALAAAYALVGSGSAPPAAAVWPATKVALATAEQVQLARQRFASGE
LEAVNQVQVAAEMPGRITRIAFESGQTVAAGQLLVQLNDAPEQALRVQLQARLRNADVVL
QRSRKLRAMNAVSQELLDNAATAVDVARGELQHVEALIAQKAIRAPFAGKLGIRRVHQGQ
YLAAGETIVSLADISQLHVNFALGEQAAPEVHAGQVLALTVDAVRGQDFQARVVAVDPVV
STARLVQVQAALPNPQGQLQPGMYAGVRLDAAQPSTVLAVPETAITYTAYGQTVFVATQD
PQHGTRVSRVQVTTGERWQGRVEITSGLTPGDRVVVSGQLKLSDGMPVEPVAQDSLQASQ
GGRQS