Protein Info for PP_2044 in Pseudomonas putida KT2440

Annotation: conserved inner membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 128 transmembrane" amino acids 33 to 57 (25 residues), see Phobius details amino acids 64 to 86 (23 residues), see Phobius details amino acids 98 to 123 (26 residues), see Phobius details PF02656: DUF202" amino acids 24 to 91 (68 residues), 63.4 bits, see alignment E=1.1e-21

Best Hits

Swiss-Prot: 42% identical to YIDH_ECO57: Inner membrane protein YidH (yidH) from Escherichia coli O157:H7

KEGG orthology group: K00389, putative membrane protein (inferred from 98% identity to ppf:Pput_3698)

Predicted SEED Role

"Inner membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L91 at UniProt or InterPro

Protein Sequence (128 amino acids)

>PP_2044 conserved inner membrane protein of unknown function (Pseudomonas putida KT2440)
MGVPEPRPDWSRRLLGQGEAPDPRFTLANERTFLAWVRTALALLGGAIAIETFAGHVLET
PLRLWLVAGLMLLSALLSAGACLRWLRVERALRRRRPLPLPALVPLLALGCLVAVLLAGL
VLWPRWHG