Protein Info for PP_2017 in Pseudomonas putida KT2440

Annotation: aminopeptidase N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 885 TIGR02414: aminopeptidase N" amino acids 13 to 884 (872 residues), 1373.8 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 71 to 192 (122 residues), 58.8 bits, see alignment E=1.6e-19 PF01433: Peptidase_M1" amino acids 233 to 445 (213 residues), 189.4 bits, see alignment E=1.4e-59 PF11940: DUF3458" amino acids 450 to 554 (105 residues), 109.3 bits, see alignment E=2.1e-35 PF17432: DUF3458_C" amino acids 559 to 885 (327 residues), 410.3 bits, see alignment E=1.4e-126

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 100% identity to ppu:PP_2017)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LB8 at UniProt or InterPro

Protein Sequence (885 amino acids)

>PP_2017 aminopeptidase N (Pseudomonas putida KT2440)
MRTEQPQVIYLKDYQAPEYLIDETHLTFELFEDHTLVHAQLVMRRNPARGAGLPPLELDG
QQLELLRASLDDQELQPGEYQLDADSLTVQPKAERFTLDTSVKIHPESNTALEGLYKSGK
MFCTQCEAEGFRKITYYLDRPDVMSTFTTTVIAEQHRYPVLLSNGNPIGSGPAEDGRHWA
TWEDPFMKPAYLFALVAGDLWCVEDSFTRQSGREVTLRIYVEPENIDKCDHAMVSLKKSM
RWDEEVYGREYDLDIFMIVAVNDFNMGAMENKGLNIFNSSCVLARAETATDAAHQRVEGV
VAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDAEFSADMNSRTVKRIEDVAYLRTHQFA
EDAGPMAHPVRPDSFIEISNFYTLTVYEKGAEVVRMVRTLLGSEGFRKGSDLYFERHDGQ
AVTTDDFIKAMEDANGVDFTQFKRWYSQAGTPRLEVSEAYDAAAQTYSLTFRQSCPQTPD
KAEKLPFVIPVELGLLDADGNDLPLQLTGEDAAQGTSRVLSVTEAEQTFTFQGIQAKPLP
SLLRGFSAPVKLSFPYDRDQLMFLMQHDSDGFNRWEAGQQLSVQVLQELIGQHQRGEALK
LDQRLITALGTVLGNVSLDPAMVAEMLSLPGEAYLTEISQVADVDAIHAAREFARQQIAE
HLFDALWARYQANREVSRSTAYVASAEHFARRSLQNIALSYLMQSGKQQVLDATLEQFEH
CDNMTERLTALAVLVNSPFEAERAKALEAFAEHFKDNPLVMDQWFSVQAASTLPGGLARV
KALMQHPAFTLKNPNKVRALIGAFAGQNLVNFHAADGSGYRFLADLVIELNALNPQIASR
QLAPLTRWRKYDDARQALMKGELERILASGELSSDVYEVVSKSLA